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'''SOLUTION STRUCTURE OF ORYZACYSTATIN-I, A CYSTEINE PROTEINASE INHIBITOR OF THE RICE, ORYZA SATIVA L. JAPONICA''' | '''SOLUTION STRUCTURE OF ORYZACYSTATIN-I, A CYSTEINE PROTEINASE INHIBITOR OF THE RICE, ORYZA SATIVA L. JAPONICA''' | ||
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[[Category: Nagata, K.]] | [[Category: Nagata, K.]] | ||
[[Category: Tanokura, M.]] | [[Category: Tanokura, M.]] | ||
[[Category: | [[Category: Alpha and beta protein]] | ||
[[Category: | [[Category: Cystatin-like fold]] | ||
[[Category: | [[Category: Cystatin/monellin superfamily]] | ||
[[Category: | [[Category: Phytocystatin family]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:24:41 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 12:24, 2 May 2008
SOLUTION STRUCTURE OF ORYZACYSTATIN-I, A CYSTEINE PROTEINASE INHIBITOR OF THE RICE, ORYZA SATIVA L. JAPONICA
Overview
The three-dimensional structure of oryzacystatin-I, a cysteine proteinase inhibitor of the rice, Oryza sativa L. japonica, has been determined in solution at pH 6.8 and 25 degrees C by (1)H and (15)N NMR spectroscopy. The main body (Glu13-Asp97) of oryzacystatin-I is well-defined and consists of an alpha-helix and a five-stranded antiparallel beta-sheet, while the N- and C-terminal regions (Ser2-Val12 and Ala98-Ala102) are less defined. The helix-sheet architechture of oryzacystatin-I is stabilized by a hydrophobic cluster formed between the alpha-helix and the beta-sheet and is considerably similar to that of monellin, a sweet-tasting protein from an African berry, as well as those of the animal cystatins studied, e.g., chicken egg white cystatin and human stefins A and B (also referred to as human cystatins A and B). Detailed structural comparison indicates that oryzacystatin-I is more similar to chicken cystatin, which belongs to the type-2 animal cystatins, than to human stefins A and B, which belong to the type-1 animal cystatins, despite different loop length.
About this Structure
1EQK is a Single protein structure of sequence from Oryza sativa japonica group. Full crystallographic information is available from OCA.
Reference
Three-dimensional solution structure of oryzacystatin-I, a cysteine proteinase inhibitor of the rice, Oryza sativa L. japonica., Nagata K, Kudo N, Abe K, Arai S, Tanokura M, Biochemistry. 2000 Dec 5;39(48):14753-60. PMID:11101290 Page seeded by OCA on Fri May 2 15:24:41 2008
