2r0h

Fungal lectin CGL3 in complex with chitotriose (chitotetraose)

spinqualitylabelsall models 2r0h, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2r0h is a 4 chain structure with sequence from Coprinus cinereus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: NAG Related: 1ulf, 1ulc, 1ulg, 1uld, 1ule, 1ul9 Gene: cgl3 (Coprinus cinereus) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [CGL3_COPCI] Binds complex carbohydrates, such as chitooligosaccharides. Does not bind lactose. May play a role in fruiting body formation. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Recent advances in genome sequencing efforts have revealed an abundance of novel putative lectins. Among these, many galectin-related proteins, characterized by many conserved residues but intriguingly lacking critical amino acids, have been found in all corners of the eukaryotic superkingdom. Here we present a structural and biochemical analysis of one representative, the galectin-related lectin CGL3 found in the inky cap mushroom Coprinopsis cinerea. This protein contains all but one conserved residues known to be involved in beta-galactoside binding in galectins. A Trp residue strictly conserved among galectins is changed to an Arg in CGL3 (R81). Accordingly, the galectin-related protein is not able to bind lactose. Screening of a glycan array revealed that CGL3 displays preference for oligomers of beta1-4-linked N-acetyl-glucosamines (chitooligosaccharides) and GalNAc beta 1-4GlcNAc (LacdiNAc). Carbohydrate-binding affinity of this novel lectin was quantified using isothermal titration calorimetry, and its mode of chitooligosaccharide coordination not involving any aromatic amino acid residues was studied by X-ray crystallography. Structural information was used to alter the carbohydrate-binding specificity and substrate affinity of CGL3. The importance of residue R81 in determining the carbohydrate-binding specificity was demonstrated by replacing this Arg with a Trp residue (R81W). This single-amino-acid change led to a lectin that failed to bind chitooligosaccharides but gained lactose binding. Our results demonstrate that, similar to the legume lectin fold, the galectin fold represents a conserved structural framework upon which dramatically altered specificities can be grafted by few alterations in the binding site and that, in consequence, many metazoan galectin-related proteins may represent lectins with novel carbohydrate-binding specificities. Structural basis for chitotetraose coordination by CGL3, a novel galectin-related protein from Coprinopsis cinerea.,Walti MA, Walser PJ, Thore S, Grunler A, Bednar M, Kunzler M, Aebi M J Mol Biol. 2008 May 23;379(1):146-59. Epub 2008 Apr 3. PMID:18440554 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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