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==Crystal structure of human WT NDRG3==
==Crystal structure of human WT NDRG3==
<StructureSection load='6l4b' size='340' side='right'caption='[[6l4b]]' scene=''>
<StructureSection load='6l4b' size='340' side='right'caption='[[6l4b]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L4B OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6L4B FirstGlance]. <br>
<table><tr><td colspan='2'>[[6l4b]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L4B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L4B FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6l4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l4b OCA], [http://pdbe.org/6l4b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l4b RCSB], [http://www.ebi.ac.uk/pdbsum/6l4b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l4b ProSAT]</span></td></tr>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NDRG3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l4b OCA], [https://pdbe.org/6l4b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l4b RCSB], [https://www.ebi.ac.uk/pdbsum/6l4b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l4b ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The N-Myc downstream-regulated gene (NDRG) family belongs to the alpha/beta-hydrolase fold and is known to exert various physiologic functions in cell proliferation, differentiation, and hypoxia-induced cancer metabolism. In particular, NDRG3 is closely related to proliferation and migration of prostate cancer cells, and recent studies reported its implication in lactate-triggered hypoxia responses or tumorigenesis. However, the underlying mechanism for the functions of NDRG3 remains unclear. Here, we report the crystal structure of human NDRG3 at 2.2 A resolution, with six molecules in an asymmetric unit. While NDRG3 adopts the alpha/beta-hydrolase fold, complete substitution of the canonical catalytic triad residues to non-reactive residues and steric hindrance around the pseudo-active site seem to disable the alpha/beta-hydrolase activity. While NDRG3 shares a high similarity to NDRG2 in terms of amino acid sequence and structure, NDRG3 exhibited remarkable structural differences in a flexible loop corresponding to helix alpha6 of NDRG2 that is responsible for tumor suppression. Thus, this flexible loop region seems to play a distinct role in oncogenic progression induced by NDRG3. Collectively, our studies could provide structural and biophysical insights into the molecular characteristics of NDRG3.
Structural and Biophysical Analyses of Human N-Myc Downstream-Regulated Gene 3 (NDRG3) Protein.,Kim KR, Kim KA, Park JS, Jang JY, Choi Y, Lee HH, Lee DC, Park KC, Yeom YI, Kim HJ, Han BW Biomolecules. 2020 Jan 6;10(1). pii: biom10010090. doi: 10.3390/biom10010090. PMID:31935861<ref>PMID:31935861</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6l4b" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Human]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Han BW]]
[[Category: Han, B W]]
[[Category: Kim KR]]
[[Category: Kim, K R]]
[[Category: Alpha/beta-hydrolase fold]]
[[Category: Ndrg3]]
[[Category: Signaling protein]]
[[Category: Unfolded helix]]
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