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==Crystal structure of lys170 CBD==
==Crystal structure of lys170 CBD==
<StructureSection load='7d55' size='340' side='right'caption='[[7d55]]' scene=''>
<StructureSection load='7d55' size='340' side='right'caption='[[7d55]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D55 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D55 FirstGlance]. <br>
<table><tr><td colspan='2'>[[7d55]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_phage_phim1ef22 Enterococcus phage phim1ef22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D55 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D55 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d55 OCA], [https://pdbe.org/7d55 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d55 RCSB], [https://www.ebi.ac.uk/pdbsum/7d55 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d55 ProSAT]</span></td></tr>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PHIM1EF22_0110 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2218498 Enterococcus phage phiM1EF22])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d55 OCA], [https://pdbe.org/7d55 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d55 RCSB], [https://www.ebi.ac.uk/pdbsum/7d55 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d55 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Lysins are a class of hydrolytic enzymes used by bacteriophages to target and cleave the peptidoglycan of bacterial cell walls during their lytic cycle. The lysins from bacteriophages that infect Gram-positive bacteria are typically monomeric and consist of one or two catalytic domains (CD) and a cell binding domain (CBD). However, multimeric lysins encoded by a single gene have also been reported, among which Lys170 from enterococcal phage F170/08 was one of the first identified. Here, we determined the crystal structure of Lys170 CBD at 1.40 A resolution. The structure reveals that Lys170 CBDs assemble into a tetrameric functional unit and that each monomer folds into a three-stranded beta-sheet core capped on each side by an alpha-helix. In addition, we identified key residues of Lys170 CBD involved in host cell binding. Our work provides a basis for designing highly efficient lysins targeting Enterococcus faecalis.
Structural and biochemical analyses of the tetrameric cell binding domain of Lys170 from enterococcal phage F170/08.,Xu X, Zhang D, Zhou B, Zhen X, Ouyang S Eur Biophys J. 2021 Feb 20. pii: 10.1007/s00249-021-01511-x. doi:, 10.1007/s00249-021-01511-x. PMID:33609147<ref>PMID:33609147</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7d55" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Enterococcus phage phim1ef22]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Zhen X]]
[[Category: Zhen, X]]
[[Category: Hydrolase]]
[[Category: Lysin]]
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