7d84: Difference between revisions
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==34-fold symmetry Salmonella S ring formed by full-length FliF== | ==34-fold symmetry Salmonella S ring formed by full-length FliF== | ||
<StructureSection load='7d84' size='340' side='right'caption='[[7d84]]' scene=''> | <StructureSection load='7d84' size='340' side='right'caption='[[7d84]], [[Resolution|resolution]] 3.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D84 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D84 FirstGlance]. <br> | <table><tr><td colspan='2'>[[7d84]] is a 34 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_typhimurium"_loeffler_1892 "bacillus typhimurium" loeffler 1892]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D84 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D84 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d84 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d84 OCA], [https://pdbe.org/7d84 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d84 RCSB], [https://www.ebi.ac.uk/pdbsum/7d84 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d84 ProSAT]</span></td></tr> | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fliF, fla AII.1, fla BI, STM1969 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d84 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d84 OCA], [https://pdbe.org/7d84 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d84 RCSB], [https://www.ebi.ac.uk/pdbsum/7d84 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d84 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[[https://www.uniprot.org/uniprot/FLIF_SALTY FLIF_SALTY]] The M ring may be actively involved in energy transduction. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The bacterial flagellar MS ring is a transmembrane complex acting as the core of the flagellar motor and template for flagellar assembly. The C ring attached to the MS ring is involved in torque generation and rotation switch, and a large symmetry mismatch between these two rings has been a long puzzle, especially with respect to their role in motor function. Here, using cryoEM structural analysis of the flagellar basal body and the MS ring formed by full-length FliF from Salmonella enterica, we show that the native MS ring is formed by 34 FliF subunits with no symmetry variation. Symmetry analysis of the C ring shows a variation with a peak at 34-fold, suggesting flexibility in C ring assembly. Finally, our data also indicate that FliF subunits assume two different conformations, contributing differentially to the inner and middle parts of the M ring and thus resulting in 23- and 11-fold subsymmetries in the inner and middle M ring, respectively. The internal core of the M ring, formed by 23 subunits, forms a hole of the right size to accommodate the protein export gate. | |||
Native flagellar MS ring is formed by 34 subunits with 23-fold and 11-fold subsymmetries.,Kawamoto A, Miyata T, Makino F, Kinoshita M, Minamino T, Imada K, Kato T, Namba K Nat Commun. 2021 Jul 9;12(1):4223. doi: 10.1038/s41467-021-24507-9. PMID:34244518<ref>PMID:34244518</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7d84" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Bacillus typhimurium loeffler 1892]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Imada K]] | [[Category: Imada, K]] | ||
[[Category: Kato T]] | [[Category: Kato, T]] | ||
[[Category: Kawamoto A]] | [[Category: Kawamoto, A]] | ||
[[Category: Kinoshita M]] | [[Category: Kinoshita, M]] | ||
[[Category: Makino F]] | [[Category: Makino, F]] | ||
[[Category: Minamino T]] | [[Category: Minamino, T]] | ||
[[Category: Miyata T]] | [[Category: Miyata, T]] | ||
[[Category: Namba K]] | [[Category: Namba, K]] | ||
[[Category: Flagellar motor]] | |||
[[Category: Membrane protein]] | |||
[[Category: Torque generation]] | |||
[[Category: Transmembrane protein complex]] | |||
[[Category: Type iii protein export system]] | |||
