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==Cytochrome P450 (CYP105D18) complex with papaverine==
==Cytochrome P450 (CYP105D18) complex with papaverine==
<StructureSection load='7dls' size='340' side='right'caption='[[7dls]]' scene=''>
<StructureSection load='7dls' size='340' side='right'caption='[[7dls]], [[Resolution|resolution]] 2.06&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DLS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DLS FirstGlance]. <br>
<table><tr><td colspan='2'>[[7dls]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_31255 Atcc 31255]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DLS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DLS FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dls OCA], [https://pdbe.org/7dls PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dls RCSB], [https://www.ebi.ac.uk/pdbsum/7dls PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dls ProSAT]</span></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EV1:1-(3,4-DIMETHOXYBENZYL)-6,7-DIMETHOXYISOQUINOLINE'>EV1</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7di3|7di3]]</div></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SLA_5925 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39478 ATCC 31255])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dls OCA], [https://pdbe.org/7dls PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dls RCSB], [https://www.ebi.ac.uk/pdbsum/7dls PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dls ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The bacterial CYP105 family is involved in secondary metabolite biosynthetic pathways and plays essential roles in the biotransformation of xenobiotics. This study investigates the newly identified H2O2-mediated CYP105D18 from Streptomyces laurentii as the first bacterial CYP for N-oxidation. The catalytic efficiency of CYP105D18 for papaverine N-oxidation was 1.43 s(-1) microM (-1). The heme oxidation rate (k) was low (&lt;0.3 min(-1)) in the presence of 200 mM H2O2. This high H2O2 tolerance capacity of CYP105D18 led to higher turnover prior to heme oxidation. Additionally, the high-resolution papaverine complexed structure and substrate-free structure of CYP105D18 were determined. Structural analysis and activity assay results revealed that CYP105D18 had a strong substrate preference for papaverine because of its bendable structure. These findings establish a basis for biotechnological applications of CYP105D18 in the pharmaceutical and medicinal industries.
Characterization of high-H2O2-tolerant bacterial cytochrome P450 CYP105D18: insights into papaverine N-oxidation.,Pardhe BD, Do H, Jeong CS, Kim KH, Lee JH, Oh TJ IUCrJ. 2021 Jun 29;8(Pt 4):684-694. doi: 10.1107/S2052252521005522. eCollection, 2021 Jul 1. PMID:34258016<ref>PMID:34258016</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7dls" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Atcc 31255]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Do H]]
[[Category: Do, H]]
[[Category: Lee JH]]
[[Category: Lee, J H]]
[[Category: Chemical modification]]
[[Category: Eukaryotic cytochrome p450]]
[[Category: Heme oxidation]]
[[Category: Papaverine n-oxide]]
[[Category: Transferase]]
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