Sandbox Reserved 1688: Difference between revisions
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There are seven conserved structural motifs, A to G, throughout all RdRps. A to E are motifs present in the palm domain. Motif G and F are part of the fingers domain. Due to HCV being a positive RNA strand, it contains an additional motif termed H. The H motif is present in the thumb domain. Motif C, a heavily conserved motif, is formed by a loop and two flanking beta strands. The loop region is essential for binding the Mg2+ ions. Motif F is comprised of a loop and a beta strand. This motif interacts with the phosphate group of an incoming NTP. In HCV it is predicted to promote RNA synthesis. Motif G is a loop that is a part of the template strand entrance tunnel in HCV. Not including motifs H, F, and G, the remaining motifs are functionally conserved in NS5B protein in regards to a traditional RdRp. | There are seven conserved structural motifs, A to G, throughout all RdRps. A to E are motifs present in the palm domain. Motif G and F are part of the fingers domain. Due to HCV being a positive RNA strand, it contains an additional motif termed H. The H motif is present in the thumb domain. Motif C, a heavily conserved motif, is formed by a loop and two flanking beta strands. The loop region is essential for binding the Mg2+ ions. Motif F is comprised of a loop and a beta strand. This motif interacts with the phosphate group of an incoming NTP. In HCV it is predicted to promote RNA synthesis. Motif G is a loop that is a part of the template strand entrance tunnel in HCV. Not including motifs H, F, and G, the remaining motifs are functionally conserved in NS5B protein in regards to a traditional RdRp. | ||
Catalytic Site – | Catalytic Site – | ||
While the fingers domain of the NS5B protein is structurally different than other RdRps, the catalytic site (largely housed in the palm region) remain structural conserved. | While the fingers domain of the NS5B protein is structurally different than other RdRps, the catalytic site (largely housed in the palm region) remain structural conserved. <scene name='NS5B/Native_ns5b/7'>active site aspartates (220, 318 and 319)</scene> | ||
</StructureSection> | </StructureSection> | ||
