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| **[[2pzb]] – BaNADS - ''Bacillus anthracis'' <br /> | | **[[2pzb]] – BaNADS - ''Bacillus anthracis'' <br /> |
| **[[3dpi]] – NADS – ''Burkholderia pseudomallei''<br /> | | **[[3dpi]] – NADS – ''Burkholderia pseudomallei''<br /> |
| | **[[6kv3]] – NADS – ''Staphylococcus aureus''<br /> |
| | **[[5wp0]] – NADS – ''Allivibrio fischeri''<br /> |
| | **[[5huh]], [[5huj]] – NADS – ''Streptococcus pyogenes''<br /> |
| | **[[3n05]] – NADS – ''Streptomyces avermitillis''<br /> |
| | **[[4xfd]] – PaNADS – ''Pseudomonas aeruginosa''<br /> |
| | **[[4q16]] – NADS – ''Deinococcus radiodurans''<br /> |
| | **[[2e18]] – NADS – ''Pyrococcus horikoshii''<br /> |
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| *NADS binary complex | | *NADS binary complex |
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| **[[1wxh]] – EcNADS + NAD <br /> | | **[[1wxh]] – EcNADS + NAD <br /> |
| **[[3hmq]] – BsNADS + NAD – ''Salmonella typhimurium'' <br /> | | **[[3hmq]] – BsNADS + NAD – ''Salmonella typhimurium'' <br /> |
| | **[[6c8q]] – NADS + NAD – ''Enterococcus faecalis''<br /> |
| | **[[5f23]] – PaNADS + NAD <br /> |
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| *NADS ternary complex | | *NADS ternary complex |
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| **[[1wxg]] – EcNADS + Mg + deamino-NAD <br /> | | **[[1wxg]] – EcNADS + Mg + deamino-NAD <br /> |
| **[[2pz8]] – BaNADS + Mg + ATP derivative <br /> | | **[[2pz8]] – BaNADS + Mg + ATP derivative <br /> |
| | **[[3fiu]] – NADS + Mg + AMP – ''Francisella tularensis''<br /> |
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| *NADS quaternary complex | | *NADS quaternary complex |
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| **[[3szg]] – MtNADS (mutant) + AMP + pyridinium derivative <br /> | | **[[3szg]] – MtNADS (mutant) + AMP + pyridinium derivative <br /> |
| **[[3syt]] – MtNADS + AMP + NAD + glutamate <br /> | | **[[3syt]] – MtNADS + AMP + NAD + glutamate <br /> |
| | **[[3dla]] – MtNADS + norleucine + deamido-NAD <br /> |
| **[[6ofc]] – MtNADS (mutant) + AMP + sulfonamide derivative + glutamate <br /> | | **[[6ofc]] – MtNADS (mutant) + AMP + sulfonamide derivative + glutamate <br /> |
| | **[[4f4h]] – NADS – ''Burkholderia thailandensis''<br /> |
| | **[[3ilv]] – NADS – ''Cytophaga hutchinsonii''<br /> |
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Function
NAD+ synthase or NH3-dependent NAD synthetase (NADS) catalyzes the conversion of ATP, deamino-NAD+ and NH3 to AMP, NAD+ and diphosphate (PP). NADS is part of the NAD synthesis pathway[1] . See NAD.
Structural highlights
The ATP binding site contains Mg+2 ion. The NAD binding site is located in the interface of the 2 subunits[2]. Water molecules are shown as red spheres.
- ↑ Suda Y, Tachikawa H, Yokota A, Nakanishi H, Yamashita N, Miura Y, Takahashi N. Saccharomyces cerevisiae QNS1 codes for NAD(+) synthetase that is functionally conserved in mammals. Yeast. 2003 Aug;20(11):995-1005. PMID:12898714 doi:https://dx.doi.org/10.1002/yea.1008
- ↑ Kang GB, Kim YS, Im YJ, Rho SH, Lee JH, Eom SH. Crystal structure of NH3-dependent NAD+ synthetase from Helicobacter pylori. Proteins. 2005 Mar 1;58(4):985-8. PMID:15645437 doi:10.1002/prot.20377
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3D Structures of NAD+ synthase
Updated on 15-November-2021
{"openlevels":0}
- NAD synthase
- NAD – NADS – Campylobacter jejuni
- 3q4g – NADS (mutant) – Vibrio cholerae
- 1wxf – EcNADS – Escherichia coli
- 1xnh – HpNADS - Helicobacter pylori
- 2pzb – BaNADS - Bacillus anthracis
- 3dpi – NADS – Burkholderia pseudomallei
- 6kv3 – NADS – Staphylococcus aureus
- 5wp0 – NADS – Allivibrio fischeri
- 5huh, 5huj – NADS – Streptococcus pyogenes
- 3n05 – NADS – Streptomyces avermitillis
- 4xfd – PaNADS – Pseudomonas aeruginosa
- 4q16 – NADS – Deinococcus radiodurans
- 2e18 – NADS – Pyrococcus horikoshii
- NADS binary complex
- 1ifx – BsNADS + deamino-NAD – Bacillus subtilis
- 1wxh – EcNADS + NAD
- 3hmq – BsNADS + NAD – Salmonella typhimurium
- 6c8q – NADS + NAD – Enterococcus faecalis
- 5f23 – PaNADS + NAD
- NADS ternary complex
- 1ih8 – BsNADS + Mg + ATP derivative
- 1wxe – EcNADS + Mg + AMP
- 1wxg – EcNADS + Mg + deamino-NAD
- 2pz8 – BaNADS + Mg + ATP derivative
- 3fiu – NADS + Mg + AMP – Francisella tularensis
- NADS quaternary complex
- 1ee1 – BsNADS + Mg + ATP + deamino-NAD
- 1fyd – BsNADS + Mg + AMP + PP
- 1kqp – BsNADS + Mg + PP + NAD derivative
- 1wxi – EcNADS + Mg + AMP + PP
- 1xng – HpNADS + Mg + ATP + deamino-NAD
- 2pza – BaNADS + Mg + AMP + PP
- NADS quinary complex
- 1nsy – BsNADS + Mg + ATP + ADP + PP
- 2nsy – BsNADS + Mg + AMP + NAD + PP
- NADS glutamine-dependent
- 6ofb – hNADS + AMP + NAD derivative
- 3sdb – MtNADS (mutant) – Mycobacterium tuberculosis
- 3seq, 3sez – MtNADS (mutant) + ATP derivative + pyridinium derivative
- 3szg – MtNADS (mutant) + AMP + pyridinium derivative
- 3syt – MtNADS + AMP + NAD + glutamate
- 3dla – MtNADS + norleucine + deamido-NAD
- 6ofc – MtNADS (mutant) + AMP + sulfonamide derivative + glutamate
- 4f4h – NADS – Burkholderia thailandensis
- 3ilv – NADS – Cytophaga hutchinsonii
References