7pbi: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==4-ethylphenol oxidase from Gulosibacter chungangensis: isoeugenol complex== | ==4-ethylphenol oxidase from Gulosibacter chungangensis: isoeugenol complex== | ||
<StructureSection load='7pbi' size='340' side='right'caption='[[7pbi]]' scene=''> | <StructureSection load='7pbi' size='340' side='right'caption='[[7pbi]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PBI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PBI FirstGlance]. <br> | <table><tr><td colspan='2'>[[7pbi]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PBI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PBI FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pbi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pbi OCA], [https://pdbe.org/7pbi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pbi RCSB], [https://www.ebi.ac.uk/pdbsum/7pbi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pbi ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=H7Y:ISOEUGENOL'>H7Y</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pbi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pbi OCA], [https://pdbe.org/7pbi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pbi RCSB], [https://www.ebi.ac.uk/pdbsum/7pbi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pbi ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The vanillyl-alcohol oxidase (VAO) family is a rich source of biocatalysts for the oxidative bioconversion of phenolic compounds. Through genome mining and sequence comparisons, we found that several family members lack a generally conserved catalytic aspartate. This finding led us to study a VAO-homolog featuring a glutamate residue in place of the common aspartate. This 4-ethylphenol oxidase from Gulosibacter chungangensis (Gc4EO) shares 42% sequence identity with VAO, contains the same 8alpha-N3-histidyl-bound FAD and uses oxygen as electron acceptor. However, Gc4EO features a distinct substrate scope and product specificity as it is primarily effective in the dehydrogenation of para -substituted phenols with little generation of hydroxylated products. The three-dimensional structure shows that the characteristic glutamate side chain creates a closely packed environment that may limit water accessibility and thereby protect from hydroxylation. With its high thermal stability, well defined structural properties and high expression yields, Gc4EO may become a catalyst of choice for the specific dehydrogenation of phenolic compounds bearing small substituents. | |||
Discovery, biocatalytic exploration and structural analysis of a 4-ethylphenol oxidase from Gulosibacter chungangensis.,Laura A, Gran-Scheuch A, Guo Y, Trajkovic M, Saifuddin M, Fraaije MW, Mattevi A Chembiochem. 2021 Sep 15. doi: 10.1002/cbic.202100457. PMID:34523783<ref>PMID:34523783</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7pbi" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Alvigini L]] | [[Category: Alvigini, L]] | ||
[[Category: Mattevi A]] | [[Category: Mattevi, A]] | ||
[[Category: 4-alkylphenol oxidase]] | |||
[[Category: 4-vinylphenol]] | |||
[[Category: Biocatalysis]] | |||
[[Category: Dehydrogenation]] | |||
[[Category: Enzyme structure]] | |||
[[Category: Flavoprotein]] | |||
[[Category: Genome mining]] | |||
[[Category: Oxidoreductase]] | |||
