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| ==X-Ray Crystal Structure of the SARS Coronavirus Main Protease== | | ==X-Ray Crystal Structure of the SARS Coronavirus Main Protease== |
| <StructureSection load='1q2w' size='340' side='right'caption='[[1q2w]], [[Resolution|resolution]] 1.86Å' scene=''> | | <StructureSection load='1q2w' size='340' side='right'caption='[[1q2w]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[1q2w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cvhsa Cvhsa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q2W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q2W FirstGlance]. <br> | | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q2W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q2W FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr> | | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q2w OCA], [https://pdbe.org/1q2w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q2w RCSB], [https://www.ebi.ac.uk/pdbsum/1q2w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q2w ProSAT]</span></td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=694009 CVHSA])</td></tr>
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| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q2w OCA], [http://pdbe.org/1q2w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q2w RCSB], [http://www.ebi.ac.uk/pdbsum/1q2w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1q2w ProSAT]</span></td></tr> | |
| </table> | | </table> |
| == Function ==
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| [[http://www.uniprot.org/uniprot/R1A_CVHSA R1A_CVHSA]] The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK (By similarity). Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> Nsp9 is a ssRNA-binding protein.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref>
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| == Evolutionary Conservation == | | == Evolutionary Conservation == |
| [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| ==See Also== | | ==See Also== |
| *[[SARS Coronavirus Main Proteinase|SARS Coronavirus Main Proteinase]] | | *[[Virus protease 3D structures|Virus protease 3D structures]] |
| == References ==
| |
| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Cvhsa]]
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| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Bonanno, J B]] | | [[Category: Bonanno JB]] |
| [[Category: Burley, S K]] | | [[Category: Burley SK]] |
| [[Category: Fowler, R]] | | [[Category: Fowler R]] |
| [[Category: Gupta, S]] | | [[Category: Gupta S]] |
| [[Category: Harris, T]] | | [[Category: Harris T]] |
| [[Category: Hendle, J]] | | [[Category: Hendle J]] |
| [[Category: Liu, E T]] | | [[Category: Liu ET]] |
| [[Category: Lorimer, D]] | | [[Category: Lorimer D]] |
| [[Category: Romero, R]] | | [[Category: Romero R]] |
| [[Category: Sauder, J M]] | | [[Category: Sauder JM]] |
| [[Category: Wei, C L]] | | [[Category: Wei CL]] |
| [[Category: Hydrolase]]
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| [[Category: Sars-cov main protease]]
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