2d4c

Crystal structure of the endophilin BAR domain mutant

spinqualitylabelsall models 2d4c, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2d4c is a 4 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CA Related: 1x03, 1x04 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [SH3G2_HUMAN] Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The crescent-shaped BAR (Bin/Amphiphysin/Rvs-homology) domain dimer is a versatile protein module that senses and generates positive membrane curvature. The BAR domain dimer of human endophilin-A1, solved at 3.1 A, has a unique structure consisting of a pair of helix-loop appendages sprouting out from the crescent. The appendage's short helices form a hydrophobic ridge, which runs across the concave surface at its center. Examining liposome binding and tubulation in vitro using purified BAR domain and its mutants indicated that the ridge penetrates into the membrane bilayer and enhances liposome tubulation. BAR domain-expressing cells exhibited marked plasma membrane tubulation in vivo. Furthermore, a swinging-arm mutant lost liposome tubulation activity yet retaining liposome binding. These data suggested that the rigid crescent dimer shape is crucial for the tubulation. We here propose that the BAR domain drives membrane curvature by coordinate action of the crescent's scaffold mechanism and the ridge's membrane insertion in addition to membrane binding via amino-terminal amphipathic helix. Endophilin BAR domain drives membrane curvature by two newly identified structure-based mechanisms.,Masuda M, Takeda S, Sone M, Ohki T, Mori H, Kamioka Y, Mochizuki N EMBO J. 2006 Jun 21;25(12):2889-97. Epub 2006 Jun 8. PMID:16763557 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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