2z6p: Difference between revisions

Latest revision as of 10:47, 8 December 2021

Crystal Structure of the Ufc1, Ufm1 conjugating enzyme 1

Structural highlights

2z6p is a 1 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:	MSE
Related:	2z6o
Gene:	UFC1 (HUMAN)
Activity:	Ubiquitin--protein ligase, with EC number 6.3.2.19
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[UFC1_HUMAN] E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Ubiquitin and ubiquitin-like protein-conjugating enzymes play central roles in posttranslational modification processes. The ubiquitin-fold modifier 1 (Ufm1), one of a variety of ubiquitin-like modifiers, is covalently attached to target proteins via Uba5 and Ufm1-conjugating enzyme 1 (Ufc1), which are analogous to the E1 and E2 ubiquitylation enzymes. As Ufm1-related proteins are conserved in metazoa and plants, the Ufm1 system likely plays important roles in various multicellular organisms. Herein, we report the X-ray structure of human Ufc1 determined at 1.6 A resolution. The Ufc1 structure comprises a canonical E2 domain and an additional N-terminal domain. The Uba5 binding site on Ufc1 was assigned by structural comparison of Ufc1 and Ubc12 and related mutational analyses. In addition, we show that the N-terminal unique domain of Ufc1 contributes to thermal stability.

Crystal structure of Ufc1, the Ufm1-conjugating enzyme.,Mizushima T, Tatsumi K, Ozaki Y, Kawakami T, Suzuki A, Ogasahara K, Komatsu M, Kominami E, Tanaka K, Yamane T Biochem Biophys Res Commun. 2007 Nov 3;362(4):1079-84. Epub 2007 Aug 30. PMID:17825256^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Komatsu M, Chiba T, Tatsumi K, Iemura S, Tanida I, Okazaki N, Ueno T, Kominami E, Natsume T, Tanaka K. A novel protein-conjugating system for Ufm1, a ubiquitin-fold modifier. EMBO J. 2004 May 5;23(9):1977-86. Epub 2004 Apr 8. PMID:15071506 doi:https://dx.doi.org/10.1038/sj.emboj.7600205
↑ Mizushima T, Tatsumi K, Ozaki Y, Kawakami T, Suzuki A, Ogasahara K, Komatsu M, Kominami E, Tanaka K, Yamane T. Crystal structure of Ufc1, the Ufm1-conjugating enzyme. Biochem Biophys Res Commun. 2007 Nov 3;362(4):1079-84. Epub 2007 Aug 30. PMID:17825256 doi:10.1016/j.bbrc.2007.08.129

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OCA

[1] Komatsu M, Chiba T, Tatsumi K, Iemura S, Tanida I, Okazaki N, Ueno T, Kominami E, Natsume T, Tanaka K. A novel protein-conjugating system for Ufm1, a ubiquitin-fold modifier. EMBO J. 2004 May 5;23(9):1977-86. Epub 2004 Apr 8. PMID:15071506 doi:https://dx.doi.org/10.1038/sj.emboj.7600205

[2] Mizushima T, Tatsumi K, Ozaki Y, Kawakami T, Suzuki A, Ogasahara K, Komatsu M, Kominami E, Tanaka K, Yamane T. Crystal structure of Ufc1, the Ufm1-conjugating enzyme. Biochem Biophys Res Commun. 2007 Nov 3;362(4):1079-84. Epub 2007 Aug 30. PMID:17825256 doi:10.1016/j.bbrc.2007.08.129

[1]

[2]

@@ Line 1: / Line 1: @@
 ==Crystal Structure of the Ufc1, Ufm1 conjugating enzyme 1==
-<StructureSection load='2z6p' size='340' side='right' caption='[[2z6p]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+<StructureSection load='2z6p' size='340' side='right'caption='[[2z6p]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2z6p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z6P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Z6P FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2z6p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z6P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z6P FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2z6o|2z6o]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2z6o|2z6o]]</div></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">UFC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">UFC1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2z6p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z6p OCA], [http://pdbe.org/2z6p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2z6p RCSB], [http://www.ebi.ac.uk/pdbsum/2z6p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2z6p ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z6p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z6p OCA], [https://pdbe.org/2z6p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z6p RCSB], [https://www.ebi.ac.uk/pdbsum/2z6p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z6p ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/UFC1_HUMAN UFC1_HUMAN]] E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage.<ref>PMID:15071506</ref>
+[[https://www.uniprot.org/uniprot/UFC1_HUMAN UFC1_HUMAN]] E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage.<ref>PMID:15071506</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 31: / Line 31: @@
 </div>
 <div class="pdbe-citations 2z6p" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Ubiquitin-fold modifier|Ubiquitin-fold modifier]]
 == References ==
 <references/>
@@ Line 36: / Line 39: @@
 </StructureSection>
 [[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Ubiquitin--protein ligase]]
 [[Category: Kawakami, T]]

2z6p: Difference between revisions

Latest revision as of 10:47, 8 December 2021

Crystal Structure of the Ufc1, Ufm1 conjugating enzyme 1

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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