Sandbox Reserved 1656: Difference between revisions
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Deubiquitinases are key enzymes belonging to the vast group of '''proteases'''.They take part in the removal of ubiquitin molecules on proteins which have been ubiquitinated, allowing the degradation of [https://en.wikipedia.org/wiki/Ubiquitin ubiquitin]-tagged proteins. As a result, these enzymes play an important role being implicated in the regulation of '''protein degradation'''. Indeed, the pathway for a protein to be degraded involves, an enzymatic cascade that will add a poly-ubiquitin fragment to the protein. This mechanism is called [https://fr.wikipedia.org/wiki/Ubiquitination ubiquitination(fr)] [https://en.wikipedia.org/wiki/Ubiquitin#Ubiquitylation (en)]. Following this step, mono or poly-ubiquitin is by deubiquitinases from the protein. | Deubiquitinases are key enzymes belonging to the vast group of '''proteases'''.They take part in the removal of ubiquitin molecules on proteins which have been ubiquitinated, allowing the degradation of [https://en.wikipedia.org/wiki/Ubiquitin ubiquitin]-tagged proteins. As a result, these enzymes play an important role being implicated in the regulation of '''protein degradation'''. Indeed, the pathway for a protein to be degraded involves, an enzymatic cascade that will add a poly-ubiquitin fragment to the protein. This mechanism is called [https://fr.wikipedia.org/wiki/Ubiquitination ubiquitination(fr)] [https://en.wikipedia.org/wiki/Ubiquitin#Ubiquitylation (en)]. Following this step, mono or poly-ubiquitin is by deubiquitinases from the protein. | ||
==== | ==== Classes ==== | ||
Deubiquitinases belong to the protease family. This family | Deubiquitinases belong to the large protease family. This family gathers into '''five classes''',all of them definded according to the nature of the amino acid composition of their active site carrying out the catalysis. It can be either : serine proteases, [https://en.wikipedia.org/wiki/Cysteine_protease cysteine proteases], acid proteases, metalloproteases, or threonine proteases. DUBs belong to only two of these families: '''metalloproteases''' and '''cysteine proteases'''. | ||
Among the cysteine proteins, four subfamilies can be described according to their catalytic domains: ubiquitin-specific proteases ( | Among the cysteine proteins, four subfamilies can be described according to their catalytic domains: ubiquitin-specific proteases (USPs),the Ubiquitin C-terminal hydrolases (UCHs),Ovarian tumore-related proteases (OTUs) and Machado-Joseph disease proteases (MJD). The deubiquitinases belonging to the family of metalloproteases all have a JAMM catalytic domain (JAB1/MPN/Mov34 metalloenzyme). | ||
Within these two families, DUBs are classified into subfamilies according to the differences in their amino acid sequences surrounding the catalytically active amino acid residues. <ref>PMID:15571815</ref> | Within these two families, DUBs are classified into subfamilies according to the differences in their amino acid sequences surrounding the catalytically active amino acid residues. <ref>PMID:15571815</ref> | ||
