Sandbox Reserved 1656: Difference between revisions

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==== Localization ====
==== Localization ====


The localization depends on the DUB we consider. However, the majority of DUBs are found in the nucleus, plasma membrane or/and in secretory and endocytic pathways. For example, in the ubiquitin-specific proteases family, the USP21 is mostly associated with microtubules and the [https://en.wikipedia.org/wiki/Centrosome centrosome]. Thus this deubiquitinase depends on all the physiological mechanisms involving the [https://en.wikipedia.org/wiki/Microtubule microtubules]. <ref>PMID:22298430</ref>
The localization depends on the DUB we consider. Nevertheless, the majority of DUBs are found in the nucleus, plasma membrane and/or in secretory and endocytic pathways. For example, in the ubiquitin-specific proteases family (UPSs), USP21 is mostly associated with microtubules and the [https://en.wikipedia.org/wiki/Centrosome centrosome]. Thus this deubiquitinase is highly dependent on all the physiological mechanisms involving the [https://en.wikipedia.org/wiki/Microtubule microtubules]. <ref>PMID:22298430</ref>


== Structure ==
== Structure ==
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==== The overall structure ====  
==== The overall structure ====  


3TMP is the catalytic domain of human deubiquitinase DUBA in complex with ubiquitin aldehyde. It is a 8 chain structure with sequence from Human. Indeed, 3TMP is made of these two macromolecules : OTU domain-containing protein 5 (also named DUBA or OTUD5) and Polyubiquitin-C, which is the ubiquitin aldehyde. It is also made of two small molecules which are the phosphoserine (SEP) and  the amino-acetaldehyde (GLZ), they are L-peptide links. <ref>PMID:22245969</ref>
3TMP is the catalytic domain of human deubiquitinase DUBA in complex with ubiquitin aldehyde. It is a 8-chain-structure with sequences from Human. Indeed, 3TMP is made of two macromolecules : OTU domain-containing protein 5 (also named DUBA or OTUD5) and Polyubiquitin-C, which is the ubiquitin aldehyde. The catalytic domain is also composed of two small molecules the phosphoserine (SEP) and  the amino-acetaldehyde (GLZ),which they are L-peptide links. <ref>PMID:22245969</ref>


=== Impact of phosphorylation on DUB activity ===
=== Impact of phosphorylation on DUB activity ===


Evidence shows that phosphorylation influences activity of the enzyme. Phosphorylated serine seems to have the most influence on the activity of the enzyme <scene name='86/868189/Ser177/1'>especially on Ser177</scene>. The phosphorylation of this nucleotide is crucial and the protein won't work if it's not.
Evidence shows that phosphorylation influences enzyme activity. Phosphorylated serine seems to have the most influence on the enzyme activity <scene name='86/868189/Ser177/1'>especially on Ser177</scene>. The phosphorylation of this nucleotide is crucial to the protein to work.
In fact, this part bends to welcome the protein to be deubiquitinased. <ref>PMID:22245969</ref>
In fact, this part bends to welcome the protein to be deubiquitinated. <ref>PMID:22245969</ref>


==== Catalytic domain ====  
==== Catalytic domain ====  
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