1g59: Difference between revisions
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'''GLUTAMYL-TRNA SYNTHETASE COMPLEXED WITH TRNA(GLU).''' | '''GLUTAMYL-TRNA SYNTHETASE COMPLEXED WITH TRNA(GLU).''' | ||
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[[Category: Vassylyev, D G.]] | [[Category: Vassylyev, D G.]] | ||
[[Category: Yokoyama, S.]] | [[Category: Yokoyama, S.]] | ||
[[Category: | [[Category: Aminoacyl-trna synthetase]] | ||
[[Category: | [[Category: Protein-rna complex]] | ||
[[Category: | [[Category: Riken structural genomics/proteomics initiative]] | ||
[[Category: | [[Category: Rsgi]] | ||
[[Category: | [[Category: Structural genomic]] | ||
[[Category: | [[Category: Transfer rna]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:09:04 2008'' | |||
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Revision as of 14:09, 2 May 2008
GLUTAMYL-TRNA SYNTHETASE COMPLEXED WITH TRNA(GLU).
Overview
Glutamyl-tRNA synthetases (GluRSs) are divided into two distinct types, with regard to the presence or absence of glutaminyl-tRNA synthetase (GlnRS) in the genetic translation systems. In the original 19-synthetase systems lacking GlnRS, the 'non-discriminating' GluRS glutamylates both tRNAGlu and tRNAGln. In contrast, in the evolved 20-synthetase systems with GlnRS, the 'discriminating' GluRS aminoacylates only tRNAGlu. Here we report the 2.4 A resolution crystal structure of a 'discriminating' GluRS.tRNAGlu complex from Thermus thermophilus. The GluRS recognizes the tRNAGlu anticodon bases via two alpha-helical domains, maintaining the base stacking. We show that the discrimination between the Glu and Gln anticodons (34YUC36 and 34YUG36, respectively) is achieved by a single arginine residue (Arg 358). The mutation of Arg 358 to Gln resulted in a GluRS that does not discriminate between the Glu and Gln anticodons. This change mimics the reverse course of GluRS evolution from anticodon 'non-dicsriminating' to 'discriminating'.
About this Structure
1G59 is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
Structural basis for anticodon recognition by discriminating glutamyl-tRNA synthetase., Sekine S, Nureki O, Shimada A, Vassylyev DG, Yokoyama S, Nat Struct Biol. 2001 Mar;8(3):203-6. PMID:11224561 Page seeded by OCA on Fri May 2 17:09:04 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Glutamate--tRNA ligase
- Single protein
- Thermus thermophilus
- Nureki, O.
- RSGI, RIKEN Structural Genomics/Proteomics Initiative.
- Sekine, S.
- Shimada, A.
- Vassylyev, D G.
- Yokoyama, S.
- Aminoacyl-trna synthetase
- Protein-rna complex
- Riken structural genomics/proteomics initiative
- Rsgi
- Structural genomic
- Transfer rna
