7pre: Difference between revisions
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==Solution structure of the NRDI domain of Nab3== | ==Solution structure of the NRDI domain of Nab3== | ||
<StructureSection load='7pre' size='340' side='right'caption='[[7pre]]' scene=''> | <StructureSection load='7pre' size='340' side='right'caption='[[7pre]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full | <table><tr><td colspan='2'>[[7pre]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PRE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PRE FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pre FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pre OCA], [https://pdbe.org/7pre PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pre RCSB], [https://www.ebi.ac.uk/pdbsum/7pre PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pre ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pre FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pre OCA], [https://pdbe.org/7pre PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pre RCSB], [https://www.ebi.ac.uk/pdbsum/7pre PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pre ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Heterodimerization of RNA binding proteins Nrd1 and Nab3 is essential to communicate the RNA recognition in the nascent transcript with the Nrd1 recognition of the Ser5-phosphorylated Rbp1 C-terminal domain in RNA polymerase II. The structure of a Nrd1-Nab3 chimera reveals the basis of heterodimerization, filling a missing gap in knowledge of this system. The free form of the Nrd1 interaction domain of Nab3 (NRID) forms a multi-state three-helix bundle that is clamped in a single conformation upon complex formation with the Nab3 interaction domain of Nrd1 (NAID). The latter domain forms two long helices that wrap around NRID, resulting in an extensive protein-protein interface that would explain the highly favorable free energy of heterodimerization. Mutagenesis of some conserved hydrophobic residues involved in the heterodimerization leads to temperature-sensitive phenotypes, revealing the importance of this interaction in yeast cell fitness. The Nrd1-Nab3 structure resembles the previously reported Rna14/Rna15 heterodimer structure, which is part of the poly(A)-dependent termination pathway, suggesting that both machineries use similar structural solutions despite they share little sequence homology and are potentially evolutionary divergent. | |||
Structural basis of Nrd1-Nab3 heterodimerization.,Chaves-Arquero B, Martinez-Lumbreras S, Camero S, Santiveri CM, Mirassou Y, Campos-Olivas R, Jimenez MA, Calvo O, Perez-Canadillas JM Life Sci Alliance. 2022 Jan 12;5(4). pii: 5/4/e202101252. doi:, 10.26508/lsa.202101252. Print 2022 Apr. PMID:35022249<ref>PMID:35022249</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7pre" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Chaves-Arquero B]] | [[Category: Chaves-Arquero, B]] | ||
[[Category: Martinez-Lumbreras S]] | [[Category: Martinez-Lumbreras, S]] | ||
[[Category: Perez-Canadillas | [[Category: Perez-Canadillas, J M]] | ||
[[Category: Heterodimerization domain]] | |||
[[Category: Nab3]] | |||
[[Category: Nns pathway]] | |||
[[Category: Transcription]] | |||
[[Category: Transcription termination]] | |||
[[Category: Yeast]] | |||
