7prp: Difference between revisions
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==Crystal Structure of the B subunit of heat labile enterotoxin LT-IIc from Escherichia coli in apo form== | ==Crystal Structure of the B subunit of heat labile enterotoxin LT-IIc from Escherichia coli in apo form== | ||
<StructureSection load='7prp' size='340' side='right'caption='[[7prp]]' scene=''> | <StructureSection load='7prp' size='340' side='right'caption='[[7prp]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PRP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PRP FirstGlance]. <br> | <table><tr><td colspan='2'>[[7prp]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PRP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PRP FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7prp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7prp OCA], [https://pdbe.org/7prp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7prp RCSB], [https://www.ebi.ac.uk/pdbsum/7prp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7prp ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7prp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7prp OCA], [https://pdbe.org/7prp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7prp RCSB], [https://www.ebi.ac.uk/pdbsum/7prp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7prp ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The heat-labile enterotoxins of Escherichia coli and cholera toxin of Vibrio cholerae are related in structure and function. Each of these oligomeric toxins are comprised of one A polypeptide and five B polypeptides. The B-subunits bind to gangliosides, which is followed by uptake into the intoxicated cell and activation of the host's adenylate cyclase by the A-subunits. There are two antigenically distinct groups of these toxins. Group I includes cholera toxin and type I heat-labile enterotoxin of E. coli; group II contains the type II heat-labile enterotoxins of E. coli. Three variants of type II toxins, designated LT-IIa, LT-IIb and LT-IIc have been described. Earlier studies revealed the crystalline structure of LT-IIb. Herein the carbohydrate binding specificity of LT-IIc B-subunits was investigated by glycosphingolipid binding studies on thin-layer chromatograms and in microtiter wells. Binding studies using a large variety of glycosphingolipids showed that LT-IIc binds with high affinity to gangliosides with a terminal Neu5Ac<3Gal or Neu5Gc<3Gal sequence, e.g. the gangliosides GM3, GD1a and Neu5Ac<3-/Neu5Gc<3-neolactotetraosylceramide and Neu5Ac<3-/Neu5Gc<3-neolactohexaosylceramide. The crystal structure of LT-IIc B-subunits alone and with bound LSTd/sialyl-lacto-N-neotetraose d pentasaccharide uncovered the molecular basis of the ganglioside recognition. These studies revealed common and unique functional structures of the type II family of heat-labile enterotoxins. | |||
Characterization of the ganglioside recognition profile of Escherichia coli heat-labile enterotoxin LT-IIc.,Zalem D, Juhas M, Terrinoni M, King-Lyons N, Lebens M, Varrot A, Connell TD, Teneberg S Glycobiology. 2021 Dec 24. pii: 6482028. doi: 10.1093/glycob/cwab133. PMID:34972864<ref>PMID:34972864</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7prp" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Varrot A]] | [[Category: Varrot, A]] | ||
[[Category: Ganglioside]] | |||
[[Category: Heat labile enterotoxin]] | |||
[[Category: Lectin]] | |||
[[Category: Sugar binding protein]] | |||
