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| ==Crystal structure of alanine racemase from Oenococcus oeni== | | ==Crystal structure of alanine racemase from Oenococcus oeni== |
| <StructureSection load='3co8' size='340' side='right' caption='[[3co8]], [[Resolution|resolution]] 1.70Å' scene=''> | | <StructureSection load='3co8' size='340' side='right'caption='[[3co8]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[3co8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Oenob Oenob]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CO8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CO8 FirstGlance]. <br> | | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CO8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CO8 FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr> | | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3co8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3co8 OCA], [https://pdbe.org/3co8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3co8 RCSB], [https://www.ebi.ac.uk/pdbsum/3co8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3co8 ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/3co8 TOPSAN]</span></td></tr> |
| <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">OEOE_0162 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=203123 OENOB])</td></tr>
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| <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alanine_racemase Alanine racemase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.1.1 5.1.1.1] </span></td></tr>
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| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3co8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3co8 OCA], [http://pdbe.org/3co8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3co8 RCSB], [http://www.ebi.ac.uk/pdbsum/3co8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3co8 ProSAT], [http://www.topsan.org/Proteins/NYSGXRC/3co8 TOPSAN]</span></td></tr> | |
| </table> | | </table> |
| == Function ==
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| [[http://www.uniprot.org/uniprot/LYSR_OENOB LYSR_OENOB]] Catalyzes the interconversion of D-lysine and L-lysine. Can also use arginine and ornithine, but not alanine.<ref>PMID:23035128</ref>
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| == Evolutionary Conservation == | | == Evolutionary Conservation == |
| [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3co8 ConSurf]. | | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3co8 ConSurf]. |
| <div style="clear:both"></div> | | <div style="clear:both"></div> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| The crystal structure of alanine racemase from Oenococcus oeni has been determined at 1.7 A resolution using the single-wavelength anomalous dispersion (SAD) method and selenium-labelled protein. The protein exists as a symmetric dimer in the crystal, with both protomers contributing to the two active sites. Pyridoxal 5'-phosphate, a cofactor, is bound to each monomer and forms a Schiff base with Lys39. Structural comparison of alanine racemase from O. oeni (Alr) with homologous family members revealed similar domain organization and cofactor binding.
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| Structure of alanine racemase from Oenococcus oeni with bound pyridoxal 5'-phosphate.,Palani K, Burley SK, Swaminathan S Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jan 1;69(Pt 1):15-9. doi: , 10.1107/S1744309112047276. Epub 2012 Dec 25. PMID:23295479<ref>PMID:23295479</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 3co8" style="background-color:#fffaf0;"></div>
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| ==See Also== | | ==See Also== |
| *[[Alanine racemase|Alanine racemase]] | | *[[Alanine racemase 3D structures|Alanine racemase 3D structures]] |
| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Alanine racemase]] | | [[Category: Large Structures]] |
| [[Category: Oenob]]
| | [[Category: Burley SK]] |
| [[Category: Burley, S K]] | | [[Category: Palani K]] |
| [[Category: Structural genomic]]
| | [[Category: Swaminathan S]] |
| [[Category: Palani, K]] | |
| [[Category: Swaminathan, S]] | |
| [[Category: Isomerase]]
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| [[Category: NYSGXRC, New York SGX Research Center for Structural Genomics]]
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| [[Category: Plp]]
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| [[Category: PSI, Protein structure initiative]]
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| [[Category: Pyridoxal phosphate]]
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| [[Category: Tim barrel]]
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