3e1s: Difference between revisions
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==Structure of an N-terminal truncation of Deinococcus radiodurans RecD2== | ==Structure of an N-terminal truncation of Deinococcus radiodurans RecD2== | ||
<StructureSection load='3e1s' size='340' side='right' caption='[[3e1s]], [[Resolution|resolution]] 2.20Å' scene=''> | <StructureSection load='3e1s' size='340' side='right'caption='[[3e1s]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3e1s]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[3e1s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"micrococcus_radiodurans"_raj_et_al._1960 "micrococcus radiodurans" raj et al. 1960]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E1S FirstGlance]. <br> | ||
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">recD ([ | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">recD ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1299 "Micrococcus radiodurans" Raj et al. 1960])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e1s OCA], [https://pdbe.org/3e1s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e1s RCSB], [https://www.ebi.ac.uk/pdbsum/3e1s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e1s ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[[ | [[https://www.uniprot.org/uniprot/RECDL_DEIRA RECDL_DEIRA]] DNA-dependent ATPase (ssDNA better than dsDNA) and ATP-dependent 5'-3' DNA helicase. Appears to move along DNA in single base steps, powered by hydrolysis of 1 molecule of ATP. Has low processivity; short (20 bp) substrates with 5'-overhangs or forked ends are the best substrates, is much less efficient on 52 or 76 bp substrates with 5'- overhangs. The presence of single-stranded DNA-binding protein (SSB) increases unwinding 4-5 fold. Has no activity on blunt DNA or DNA with 3'-overhangs. Requires at least 10 bases of 5'-ssDNA for helicase activity.<ref>PMID:15466873</ref> <ref>PMID:19490894</ref> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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==See Also== | ==See Also== | ||
*[[Exonuclease|Exonuclease]] | *[[Exonuclease 3D structures|Exonuclease 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Micrococcus radiodurans raj et al. 1960]] | [[Category: Micrococcus radiodurans raj et al. 1960]] | ||
[[Category: Large Structures]] | |||
[[Category: Cook, N]] | [[Category: Cook, N]] | ||
[[Category: Court, R]] | [[Category: Court, R]] | ||
