6u75: Difference between revisions
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==Crystal Structure of S. Cerevisiae SUMO E3 Ligase SIZ2== | ==Crystal Structure of S. Cerevisiae SUMO E3 Ligase SIZ2== | ||
<StructureSection load='6u75' size='340' side='right'caption='[[6u75]]' scene=''> | <StructureSection load='6u75' size='340' side='right'caption='[[6u75]], [[Resolution|resolution]] 2.63Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U75 OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[6u75]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U75 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U75 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NFI1, SIZ2, YOR156C ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u75 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u75 OCA], [https://pdbe.org/6u75 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u75 RCSB], [https://www.ebi.ac.uk/pdbsum/6u75 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u75 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[[https://www.uniprot.org/uniprot/SIZ2_YEAST SIZ2_YEAST]] May act as an E3 ligase mediating SUMO/Smt3 attachment to septins. May be involved in chromosome maintenance.<ref>PMID:11333221</ref> <ref>PMID:12761287</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Repair of DNA double-stranded breaks by homologous recombination (HR) is dependent on DNA end resection and on post-translational modification of repair factors. In budding yeast, single-stranded DNA is coated by replication protein A (RPA) following DNA end resection, and DNA-RPA complexes are then SUMO-modified by the E3 ligase Siz2 to promote repair. Here, we show using enzymatic assays that DNA duplexes containing 3' single-stranded DNA overhangs increase the rate of RPA SUMO modification by Siz2. The SAP domain of Siz2 binds DNA duplexes and makes a key contribution to this process as highlighted by models and a crystal structure of Siz2 and by assays performed using protein mutants. Enzymatic assays performed using DNA that can accommodate multiple RPA proteins suggest a model in which the SUMO-RPA signal is amplified by successive rounds of Siz2-dependent SUMO modification of RPA and dissociation of SUMO-RPA at the junction between single- and double-stranded DNA. Our results provide insights on how DNA architecture scaffolds a substrate and E3 ligase to promote SUMO modification in the context of DNA repair. | |||
DNA asymmetry promotes SUMO modification of the single-stranded DNA-binding protein RPA.,Cappadocia L, Kochanczyk T, Lima CD EMBO J. 2021 Nov 15;40(22):e103787. doi: 10.15252/embj.2019103787. Epub 2021 Sep , 29. PMID:34585421<ref>PMID:34585421</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6u75" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Baker's yeast]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Cappadocia L]] | [[Category: Cappadocia, L]] | ||
[[Category: Lima | [[Category: Lima, C D]] | ||
[[Category: Ligase]] | |||
[[Category: Metal-binding]] | |||
[[Category: Nucleus]] | |||
[[Category: Phosphoprotein]] | |||
[[Category: Pia]] | |||
[[Category: Replication]] | |||
[[Category: Ring e3]] | |||
[[Category: Signal transduction]] | |||
[[Category: Siz]] | |||
[[Category: Sumo]] | |||
[[Category: Ubc9]] | |||
[[Category: Ubiquitin]] | |||
[[Category: Ubl conjugation pathway]] | |||
[[Category: Zinc-finger]] | |||
Revision as of 10:45, 16 February 2022
Crystal Structure of S. Cerevisiae SUMO E3 Ligase SIZ2
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