Sandbox Reserved 1711: Difference between revisions
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=== Domains === | === Domains === | ||
The <scene name='90/ | The <scene name='90/904316/Grd_domains/1'>GRD domain</scene> of Neurofibromin, specifically the arginine finger (R1276), binds to the Ras + GTP complex. | ||
=== Key Players === | === Key Players === | ||
An <scene name='90/905640/Arg_finger/1'>R1276</scene> (R1276) <scene name='90/904316/Arg_finger/1'>R1276</scene> present in the GRD is critical for Ras binding and is only accessible when the GRD and Sec14-PH domains are rotated in such a way that there is no steric hindrance from the surrounding dimer chains. The Closed conformation is stabilized by a <scene name='90/904316/Closed_triade/1'>triade</scene> of residues that are coordinated with transition metal-binding sites with zinc. Here, the GRD and Sec14-PH domains are oriented in a way that the H1558 and H1576 are able to interact with C1032 and form a transition binding-site with zinc. This binding site stabilizes the closed conformation and prevents Ras from associating with the GRD based on the location of the GRD in relation to the rest of the protein. | An <scene name='90/905640/Arg_finger/1'>R1276</scene> (R1276) <scene name='90/904316/Arg_finger/1'>R1276</scene> present in the GRD is critical for Ras binding and is only accessible when the GRD and Sec14-PH domains are rotated in such a way that there is no steric hindrance from the surrounding dimer chains. The Closed conformation is stabilized by a <scene name='90/904316/Closed_triade/1'>triade</scene> of residues that are coordinated with transition metal-binding sites with zinc. Here, the GRD and Sec14-PH domains are oriented in a way that the H1558 and H1576 are able to interact with C1032 and form a transition binding-site with zinc. This binding site stabilizes the closed conformation and prevents Ras from associating with the GRD based on the location of the GRD in relation to the rest of the protein. | ||
