5ook

Structure of A. marina Phycocyanin contains overlapping isoforms

spinqualitylabelsall models 5ook, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5ook is a 2 chain structure with sequence from Acaryochloris marina. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CYC, PEG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The major light harvesting antenna in all cyanobacterial species is the phycobilisome (PBS). The smallest PBS identified to date is that of Acaryochloris marina (A. marina), composed of a single four-hexamer rod. We have determined the crystal structure of phycocyanin (AmPC), the major component of the A. marina PBS (AmPBS) to 2.1A. The basic unit of the AmPC is a heterodimer of two related subunits (alpha and beta), and we show that the asymmetric unit contains a superposition of two alpha and two beta isoforms, the products of the simultaneous expression of different genes. This is the first time to our knowledge that isolated proteins crystallized with such identifiable heterogeneity. We believe that the presence of the different isoforms allows the AmPBS to have a significant bathochromic shift in its fluorescence emission spectrum, allowing, in the total absence of allophycocyanin, a better overlap with absorption of the chlorophyll d-containing reaction centers. We show that this bathochromic shift exists in intact AmPBS as well as in its disassembled components, thus suggesting that AmPC can efficiently serve as the AmPBS terminal emitter. Structural heterogeneity leads to functional homogeneity in A. marina phycocyanin.,Bar-Zvi S, Lahav A, Harris D, Niedzwiedzki DM, Blankenship RE, Adir N Biochim Biophys Acta. 2018 Jul;1859(7):544-553. doi:, 10.1016/j.bbabio.2018.04.007. Epub 2018 Apr 25. PMID:29704497 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Phycocyanin

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