6gwk

The crystal structure of Hfq from Caulobacter crescentus

spinqualitylabelsall models 6gwk, resolution 2.15Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6gwk is a 24 chain structure with sequence from Cauvc. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: hfq, CC_1745 (CAUVC) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [HFQ_CAUVC] RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs. Publication Abstract from PubMed We have solved the X-ray crystal structure of the RNA chaperone protein Hfq from the alpha-proteobacterium Caulobacter crescentus to 2.15-A resolution, resolving the conserved core of the protein and the entire C-terminal domain (CTD). The structure reveals that the CTD of neighboring hexamers pack in crystal contacts, and that the acidic residues at the C-terminal tip of the protein interact with positive residues on the rim of Hfq, as has been recently proposed for a mechanism of modulating RNA binding. De novo computational models predict a similar docking of the acidic tip residues against the core of Hfq. We also show that C. crescentus Hfq has sRNA binding and RNA annealing activities and is capable of facilitating the annealing of certain Escherichia coli sRNA:mRNA pairs in vivo. Finally, we describe how the Hfq CTD and its acidic tip residues provide a mechanism to modulate annealing activity and substrate specificity in various bacteria. Caulobacter crescentus Hfq structure reveals a conserved mechanism of RNA annealing regulation.,Santiago-Frangos A, Frohlich KS, Jeliazkov JR, Malecka EM, Marino G, Gray JJ, Luisi BF, Woodson SA, Hardwick SW Proc Natl Acad Sci U S A. 2019 May 10. pii: 1814428116. doi:, 10.1073/pnas.1814428116. PMID:31076551 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Protein Hfq

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