3tpf

Crystal structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni subsp. jejuni NCTC 11168

spinqualitylabelsall models 3tpf, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3tpf is a 6 chain structure with sequence from Camje. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: PEG NonStd Res: MSE Related: 1c9y Gene: argF, Cj0994c (CAMJE) Activity: Ornithine carbamoyltransferase, with EC number 2.1.3.3 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [OTC_CAMJE] Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline (By similarity).[HAMAP-Rule:MF_01109] Publication Abstract from PubMed Anabolic ornithine transcarbamoylase (aOTC) catalyzes the reaction between carbamoyl phosphate (CP) and L-ornithine (ORN) to form L-citrulline and phosphate in the urea cycle and L-arginine biosynthesis. The crystal structure of unliganded aOTC from Campylobacter jejuni (Cje aOTC) was determined at 2.7 A resolution and refined to an R(work) of 20.3% and an R(free) of 24.0%. Cje aOTC is a trimer that forms a head-to-head pseudohexamer in the asymmetric unit. Each monomer is composed of an N-terminal CP-binding domain and a C-terminal ORN-binding domain joined by two interdomain helices. The Cje aOTC structure presents an open conformation of the enzyme with a relatively flexible orientation of the ORN-binding domain respective to the CP-binding domain. The conformation of the B2-H3 loop (residues 68-78), which is involved in binding CP in an adjacent subunit of the trimer, differs from that seen in homologous proteins with CP bound. The loop containing the ORN-binding motif (DxxxSMG, residues 223-230) has a conformation that is different from those observed in unliganded OTC structures from other species, but is similar to those in structures with bound ORN analogs. The major differences in tertiary structure between Cje aOTC and human aOTC are described. Structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni at 2.7 A resolution.,Shabalin IG, Porebski PJ, Cooper DR, Grabowski M, Onopriyenko O, Grimshaw S, Savchenko A, Chruszcz M, Minor W Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Sep;68(Pt 9):1018-24. Epub, 2012 Aug 29. PMID:22949186 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ornithine carbamoyltransferase 3D structures

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