Sandbox Reserved 1700: Difference between revisions

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</jmol>

Another motif found in most, but not all, A family GPCR’s is the PIF motif. The three residues are found on transmembrane helices 5, 3, and 6, respectively. In MRGPRX2, the PIF motif is changed to LLF residues. '''Figure 3''' shows the conserved PIF motif on 5HT2AR, compared to the LLF motif on MRGPRX2, found at <scene name='90/904305/Llf_motif/3'>Leu-117, Leu-194, and Phe-232</scene> on transmembrane helices 5, 3, and 6, respectively. This contributes to shifting helix 6 towards helix 3, and contributes to the tighter packing of helices <ref name="Cao">PMID: 34789874</ref> <ref name="Yang">PMID: 34789875</ref> and therefore a more surface-level ligand binding site.

Another motif found in most, but not all, A family GPCR’s is the PIF motif. The three residues are found on transmembrane helices 5, 3, and 6, respectively. In MRGPRX2, the PIF motif is changed to LLF residues. '''Figure 3''' shows the conserved PIF motif on 5HT2AR, compared to the LLF motif on MRGPRX2, found at <scene name='90/904305/Llf_motif/5'>Leu-117, Leu-194, and Phe-232</scene> on transmembrane helices 5, 3, and 6, respectively. This contributes to shifting helix 6 towards helix 3, and contributes to the tighter packing of helices <ref name="Cao">PMID: 34789874</ref> <ref name="Yang">PMID: 34789875</ref> and therefore a more surface-level ligand binding site.

[[Image:PIF_resized.png|500px|center|thumb|'''Figure 3.''' Conserved PIF motif in 5HT2AR (teal) compared to the LLF motif found in MRGPRX2 (red). Transmembrane helices and residues are numbered and labeled to show how this structural change shifts the orientation of the helices.]]

Sandbox Reserved 1700: Difference between revisions

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 </jmol>
-Another motif found in most, but not all, A family GPCR’s is the PIF motif. The three residues are found on transmembrane helices 5, 3, and 6, respectively. In MRGPRX2, the PIF motif is changed to LLF residues. '''Figure 3''' shows the conserved PIF motif on 5HT2AR, compared to the LLF motif on MRGPRX2, found at <scene name='90/904305/Llf_motif/3'>Leu-117, Leu-194, and Phe-232</scene> on transmembrane helices 5, 3, and 6, respectively. This contributes to shifting helix 6 towards helix 3, and contributes to the tighter packing of helices <ref name="Cao">PMID: 34789874</ref> <ref name="Yang">PMID: 34789875</ref> and therefore a more surface-level ligand binding site.
+Another motif found in most, but not all, A family GPCR’s is the PIF motif. The three residues are found on transmembrane helices 5, 3, and 6, respectively. In MRGPRX2, the PIF motif is changed to LLF residues. '''Figure 3''' shows the conserved PIF motif on 5HT2AR, compared to the LLF motif on MRGPRX2, found at <scene name='90/904305/Llf_motif/5'>Leu-117, Leu-194, and Phe-232</scene> on transmembrane helices 5, 3, and 6, respectively. This contributes to shifting helix 6 towards helix 3, and contributes to the tighter packing of helices <ref name="Cao">PMID: 34789874</ref> <ref name="Yang">PMID: 34789875</ref> and therefore a more surface-level ligand binding site.
 [[Image:PIF_resized.png|500px|center|thumb|'''Figure 3.''' Conserved PIF motif in 5HT2AR (teal) compared to the LLF motif found in MRGPRX2 (red). Transmembrane helices and residues are numbered and labeled to show how this structural change shifts the orientation of the helices.]]