BASIL2022GV3HDT: Difference between revisions
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===='''Coupled kinase assay'''==== | ===='''Coupled kinase assay'''==== | ||
Coupled kinase assay diagram (left) with enzymes shown in color and phosphates in yellow. Phosphorylation of dCMP is measured indirectly through the conversion of NADH to NAD+. Background hydrolysis of NADH is measured and subtracted from the conversion rate in the presence of dCMP to produce specific activities (right). | |||
[[Image:Coupled assay.png | | |||
[[Image:Coupled assay.png |350px|center| tumb]] | |||
