BASIL2022GV3HDT: Difference between revisions

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== Introduction ==

Kinases (or phosphotransferases) facilitate the transfer of a phosphate group from one molecule to another, and are involved in cell growth and signaling. This work characterizes a protein (PDB ID 3HDT) with unknown function, and tests for kinase activity. In silico tools including BLASTp, Pfam, SPRITE, DALI, DeepFRI, and PyRx were used to hypothesize the function and taxonomy of 3HDT. These results indicated the protein may be a cytidylate kinase (EC: 2.7.4.X) that transfers a phosphate from ATP to dCMP. E. coli. were transformed with a plasmid containing the gene for 3HDT. The protein was then overexpressed, purified and quantified. SDS-PAGE was used to verify the purity of the protein. Kinetics assays were carried out using dCMP as a substrate, showing only limited activity. Future research should focus on other nucleotide substrates such as TMP or GMP, which also showed high affinities in docking simulations.

Kinases (or phosphotransferases) facilitate the transfer of a phosphate group from one molecule to another and are involved in cell growth and signaling. This work characterizes a protein (PDB ID 3HDT) with unknown function, and tests for kinase activity. The goal of this research is to characterize the protein, 3HDT, that has an unknown function. This research is part of the BASIL project that involved performing in silico and in vitro modules to make predictions and study the function of this protein.

OR

The goal of this research is to characterize the protein, 3HDT, that has an unknown function. This research is part of the BASIL project that involved performing in silico and in vitro modules to make predictions and study the function of this protein.

BASIL2022GV3HDT: Difference between revisions

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 == Introduction ==
-Kinases (or phosphotransferases) facilitate the transfer of a phosphate group from one molecule to another, and are involved in cell growth and signaling. This work characterizes a protein (PDB ID 3HDT) with unknown function, and tests for kinase activity. In silico tools including BLASTp, Pfam, SPRITE, DALI, DeepFRI, and PyRx were used to hypothesize the function and taxonomy of 3HDT. These results indicated the protein may be a cytidylate kinase (EC: 2.7.4.X) that transfers a phosphate from ATP to dCMP. E. coli. were transformed with a plasmid containing the gene for 3HDT. The protein was then overexpressed, purified and quantified. SDS-PAGE was used to verify the purity of the protein. Kinetics assays were carried out using dCMP as a substrate, showing only limited activity. Future research should focus on other nucleotide substrates such as TMP or GMP, which also showed high affinities in docking simulations.
+Kinases (or phosphotransferases) facilitate the transfer of a phosphate group from one molecule to another and are involved in cell growth and signaling. This work characterizes a protein (PDB ID 3HDT) with unknown function, and tests for kinase activity. The goal of this research is to characterize the protein, 3HDT, that has an unknown function. This research is part of the BASIL project that involved performing in silico and in vitro modules to make predictions and study the function of this protein.
-OR
-The goal of this research is to characterize the protein, 3HDT, that has an unknown function. This research is part of the BASIL project that involved performing in silico and in vitro modules to make predictions and study the function of this protein.