Sandbox Reserved 1722: Difference between revisions

Line 20:

==== ''Toggle Switch'' ====

In β2AR, and other Class A GPCRs, a “toggle switch” of <scene name='90/904327/B2artoggleswitchyes/7'>Trp-286</scene> which limits the proximity of the TM helices as tryptophan sterically occludes tight interaction. This results in a deep binding pocket for ligand binding. In contrast, in MRGPRX2 Trp-286 is replaced by <scene name='90/904327/Toggle_switch_gly_pt_2/1'>Gly-236</scene> <ref name="Cao"/> <ref name="Yang"/>. Glycine is a much smaller amino acid and thus allows the helices to close the base of the binding pocket. This causes MRGPRX2 to have a much shallower binding site and allows more promiscuous ligand binding. This can be seen in a shorter distance from the toggle switch to the ligand in β2AR compared to MRGPRX2.

In β2AR, and other Class A GPCRs, a “toggle switch” of <scene name='90/904327/B2artoggleswitchyes/7'>Trp-286</scene> which limits the proximity of the TM helices as tryptophan sterically occludes tight interaction. This results in a deep binding pocket for ligand binding. In contrast, in MRGPRX2 Trp-286 is replaced by <scene name='90/904327/Toggle_switch_gly_pt_2/2'>Gly-236</scene> <ref name="Cao"/> <ref name="Yang"/>. Glycine is a much smaller amino acid and thus allows the helices to close the base of the binding pocket. This causes MRGPRX2 to have a much shallower binding site and allows more promiscuous ligand binding. This can be seen in a shorter distance from the toggle switch to the ligand in β2AR compared to MRGPRX2.

==== ''Disulfide bonds'' ====

Sandbox Reserved 1722: Difference between revisions

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 20: / Line 20: @@
 ==== ''Toggle Switch'' ====
-In β2AR, and other Class A GPCRs, a “toggle switch” of <scene name='90/904327/B2artoggleswitchyes/7'>Trp-286</scene> which limits the proximity of the TM helices as tryptophan sterically occludes tight interaction. This results in a deep binding pocket for ligand binding. In contrast, in MRGPRX2 Trp-286 is replaced by <scene name='90/904327/Toggle_switch_gly_pt_2/1'>Gly-236</scene> <ref name="Cao"/> <ref name="Yang"/>. Glycine is a much smaller amino acid and thus allows the helices to close the base of the binding pocket. This causes MRGPRX2 to have a much shallower binding site and allows more promiscuous ligand binding. This can be seen in a shorter distance from the toggle switch to the ligand in β2AR compared to MRGPRX2.
+In β2AR, and other Class A GPCRs, a “toggle switch” of <scene name='90/904327/B2artoggleswitchyes/7'>Trp-286</scene> which limits the proximity of the TM helices as tryptophan sterically occludes tight interaction. This results in a deep binding pocket for ligand binding. In contrast, in MRGPRX2 Trp-286 is replaced by <scene name='90/904327/Toggle_switch_gly_pt_2/2'>Gly-236</scene> <ref name="Cao"/> <ref name="Yang"/>. Glycine is a much smaller amino acid and thus allows the helices to close the base of the binding pocket. This causes MRGPRX2 to have a much shallower binding site and allows more promiscuous ligand binding. This can be seen in a shorter distance from the toggle switch to the ligand in β2AR compared to MRGPRX2.
 ==== ''Disulfide bonds'' ====