BASIL2022GV3HDT: Difference between revisions
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[[Image:Coupled assay.png |400px|left| tumb | representation of the reaction of the coupled assay with 3HDT]] | [[Image:Coupled assay.png |400px|left| tumb | representation of the reaction of the coupled assay with 3HDT]] | ||
[[Image:Coupled assay results.png |500px|center| thumb]] | [[Image:Coupled assay results.png |500px|center| thumb | Table of data showing all eight coupled kinase assays with 3HDT]] | ||
Coupled kinase assay diagram (left) with enzymes shown in color and phosphates in yellow. Phosphorylation of dCMP is measured indirectly through the conversion of NADH to NAD+. Background hydrolysis of NADH is measured and subtracted from the conversion rate in the presence of dCMP to produce specific activities (right). | Coupled kinase assay diagram (left) with enzymes shown in color and phosphates in yellow. Phosphorylation of dCMP is measured indirectly through the conversion of NADH to NAD+. Background hydrolysis of NADH is measured and subtracted from the conversion rate in the presence of dCMP to produce specific activities (right). | ||
