BASIL2022GV3R8E: Difference between revisions
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Like many proteins with solved crystal structures, protein 3r8e has an uncharacterized and unconfirmed function. Besides the relationship with bacteria in soil, the role of this protein is still undetermined. As apart of a research project under the Biochemistry Authentic Scientific Inquiry Laboratory (BASIL) consortium, our group was tasked with characterizing and identifying the function of this protein to potentially provide further insight of the protein's relationship to the bacteria. Below is the general workflow of how we got our results. | Like many proteins with solved crystal structures, protein 3r8e has an uncharacterized and unconfirmed function. Besides the relationship with bacteria in soil, the role of this protein is still undetermined. As apart of a research project under the Biochemistry Authentic Scientific Inquiry Laboratory (BASIL) consortium, our group was tasked with characterizing and identifying the function of this protein to potentially provide further insight of the protein's relationship to the bacteria. Below is the general workflow of how we got our results. | ||
[[Image:Workflow1|500px| | [[Image:Workflow1.png|500px|]] | ||
== Methods == | == Methods == | ||
As you can see in the workflow portion above, we used a variety of in silico tools such as BLASTp, Pfam, DALI, PyRx, and PyMol to help us generate a hypothesis for our uncharacterized proteins function. Using the FASTA sequence found in the Protein Data Bank file, we then were able to find similarities between 3r8e and other characterized proteins. While exploring the DALI database, a significant structural alignment hit was found with protein 3vov. Alignment and highly conserved amino acids can be seen below. | As you can see in the workflow portion above, we used a variety of in silico tools such as BLASTp, Pfam, DALI, PyRx, and PyMol to help us generate a hypothesis for our uncharacterized proteins function. Using the FASTA sequence found in the Protein Data Bank file, we then were able to find similarities between 3r8e and other characterized proteins. While exploring the DALI database, a significant structural alignment hit was found with protein 3vov. Alignment and highly conserved amino acids can be seen below. | ||
