BASIL2022GV3HDT: Difference between revisions
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To transform our BL21-Gold E.Coli with the gene for 3HDT, we used a pMCSG19 vector with ampicillin resistance. This was digested the plasmid with Xho1, and Nde1 and ran gel electrophoresis to verify its identity. | To transform our BL21-Gold E.Coli with the gene for 3HDT, we used a pMCSG19 vector with ampicillin resistance. This was digested the plasmid with Xho1, and Nde1 and ran gel electrophoresis to verify its identity. | ||
[[Image:Plasmidmap3hdt. | [[Image:Plasmidmap3hdt.PNG |400px|left| thumb | Vector map for pMCSG19.]] | ||
[[Image:PlasmiddigestIMGJRAF.PNG |400px|left| thumb | Lane one contains ladder. Lane two contains pDNA without enzyme. Lane three contains pDNA + Xho1. Lane four contains pDNA + Nde1. Lane 5 contains pDNA + Xho1 + Nde1.]] | |||
===='''Coupled kinase assay'''==== | ===='''Coupled kinase assay'''==== | ||
