Structural highlights
Function
[FLA_CAMJ8] Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.
Publication Abstract from PubMed
Vertebrates, from zebra fish to humans, have an innate immune recognition of many bacterial flagellins. This involves a conserved eight-amino acid epitope in flagellin recognized by the Toll-like receptor 5 (TLR5). Several important human pathogens, such as Helicobacter pylori and Campylobacter jejuni, have escaped TLR5 activation by mutations in this epitope. When such mutations were introduced into Salmonella flagellin, motility was abolished. It was previously argued, using very low-resolution cryoelectron microscopy (cryo-EM), that C. jejuni accommodated these mutations by forming filaments with 7 protofilaments, rather than the 11 found in other bacteria. We have now determined the atomic structure of the C. jejuni G508A flagellar filament from a 3.5-A-resolution cryo-EM reconstruction, and show that it has 11 protofilaments. The residues in the C. jejuni TLR5 epitope have reduced contacts with the adjacent subunit compared to other bacterial flagellar filament structures. The weakening of the subunit-subunit interface introduced by the mutations in the TLR5 epitope is compensated for by extensive interactions between the outer domains of the flagellin subunits. In other bacteria, these outer domains can be nearly absent or removed without affecting motility. Furthermore, we provide evidence for the stabilization of these outer domain interactions through glycosylation of key residues. These results explain the essential role of glycosylation in C. jejuni motility, and show how the outer domains have evolved to play a role not previously found in other bacteria.
Atomic structure of the Campylobacter jejuni flagellar filament reveals how epsilon Proteobacteria escaped Toll-like receptor 5 surveillance.,Kreutzberger MAB, Ewing C, Poly F, Wang F, Egelman EH Proc Natl Acad Sci U S A. 2020 Jul 21;117(29):16985-16991. doi:, 10.1073/pnas.2010996117. Epub 2020 Jul 8. PMID:32641510[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kreutzberger MAB, Ewing C, Poly F, Wang F, Egelman EH. Atomic structure of the Campylobacter jejuni flagellar filament reveals how epsilon Proteobacteria escaped Toll-like receptor 5 surveillance. Proc Natl Acad Sci U S A. 2020 Jul 21;117(29):16985-16991. doi:, 10.1073/pnas.2010996117. Epub 2020 Jul 8. PMID:32641510 doi:https://dx.doi.org/10.1073/pnas.2010996117
