BASIL2022GV3HDT: Difference between revisions
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Beginning with BLASTp<ref>National Center for Biotechnology Information (NCBI)[Internet]. Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information; [1988] – [cited 2022 April 23].</ref>, we queried the FASTA sequence for our protein, PDB ID 3HDT. Our query sequence aligned with the NK superfamily and showed a high degree of overlap indicating that this protein is likely a member. The importance of this is that the cytidylate kinase family is a member of the NK superfamily, supporting our hypothesis that 3HDT is a cytidylate kinase. Additionally, the query hits showed several cytidylate kinase-like family proteins almost completely aligning with our protein, 3HDT. | Beginning with BLASTp<ref>National Center for Biotechnology Information (NCBI)[Internet]. Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information; [1988] – [cited 2022 April 23].</ref>, we queried the FASTA sequence for our protein, PDB ID 3HDT. Our query sequence aligned with the NK superfamily and showed a high degree of overlap indicating that this protein is likely a member. The importance of this is that the cytidylate kinase family is a member of the NK superfamily, supporting our hypothesis that 3HDT is a cytidylate kinase. Additionally, the query hits showed several cytidylate kinase-like family proteins almost completely aligning with our protein, 3HDT. | ||
[[Image:BLASTp image 1.png |600px| left | thumb | BLASTp | [[Image:BLASTp image 1.png |600px| left | thumb | BLASTp alignment showing a hit with cytidylate kinase-like family, part of the NK superfamily.]] | ||
[[Image:BLASTp image 2.png |600px| left | thumb | Query hits 1-2 show proteins with unknown function while 3-6 match a cytidylate kinase-like family protein.]] | [[Image:BLASTp image 2.png |600px| left | thumb | Query hits 1-2 show proteins with unknown function while 3-6 match a cytidylate kinase-like family protein.]] | ||
