BASIL2022GV3HDT: Difference between revisions
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[[Image:Coupled assay.png |400px|left| thumb | Coupled kinase assay diagram with enzymes shown in color and phosphates in yellow. Phosphorylation of dCMP is measured indirectly through the conversion of NADH to NAD+.]] | [[Image:Coupled assay.png |400px|left| thumb | Coupled kinase assay diagram with enzymes shown in color and phosphates in yellow. Phosphorylation of dCMP is measured indirectly through the conversion of NADH to NAD+.]] | ||
[[Image:Updated Kinetics Table 5.4.22 3.PNG |500px|center| thumb | Specific activity values and other data from all coupled kinase assays with 3HDT and dCMP.]] | [[Image:Updated Kinetics Table 5.4.22 3.PNG |500px|center| thumb | Specific activity values and other data from all coupled kinase assays with 3HDT and dCMP. Background hydrolysis of NADH was measured and subtracted from the conversion rate in the presence of dCMP before calculating specific activity values.]] | ||
