7nyq: Difference between revisions
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==Crystal structure of the Mei-P26 NHL domain== | ==Crystal structure of the Mei-P26 NHL domain== | ||
<StructureSection load='7nyq' size='340' side='right'caption='[[7nyq]]' scene=''> | <StructureSection load='7nyq' size='340' side='right'caption='[[7nyq]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NYQ FirstGlance]. <br> | <table><tr><td colspan='2'>[[7nyq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NYQ FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nyq OCA], [https://pdbe.org/7nyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nyq RCSB], [https://www.ebi.ac.uk/pdbsum/7nyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nyq ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nyq OCA], [https://pdbe.org/7nyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nyq RCSB], [https://www.ebi.ac.uk/pdbsum/7nyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nyq ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The TRIM-NHL protein Meiotic P26 (Mei-P26) acts as a regulator of cell fate in Drosophila Its activity is critical for ovarian germline stem cell maintenance, differentiation of oocytes, and spermatogenesis. Mei-P26 functions as a post-transcriptional regulator of gene expression; however, the molecular details of how its NHL domain selectively recognizes and regulates its mRNA targets have remained elusive. Here, we present the crystal structure of the Mei-P26 NHL domain at 1.6 A resolution and identify key amino acids that confer substrate specificity and distinguish Mei-P26 from closely related TRIM-NHL proteins. Furthermore, we identify mRNA targets of Mei-P26 in cultured Drosophila cells and show that Mei-P26 can act as either a repressor or activator of gene expression on different RNA targets. Our work reveals the molecular basis of RNA recognition by Mei-P26 and the fundamental functional differences between otherwise very similar TRIM-NHL proteins. | |||
Molecular insights into RNA recognition and gene regulation by the TRIM-NHL protein Mei-P26.,Salerno-Kochan A, Horn A, Ghosh P, Nithin C, Koscielniak A, Meindl A, Strauss D, Krutyholowa R, Rossbach O, Bujnicki JM, Gaik M, Medenbach J, Glatt S Life Sci Alliance. 2022 May 5;5(8). pii: 5/8/e202201418. doi:, 10.26508/lsa.202201418. Print 2022 Aug. PMID:35512835<ref>PMID:35512835</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7nyq" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Gaik M]] | [[Category: Gaik, M]] | ||
[[Category: Glatt S]] | [[Category: Glatt, S]] | ||
[[Category: Medenbach J]] | [[Category: Medenbach, J]] | ||
[[Category: Salerno-Kochan A]] | [[Category: Salerno-Kochan, A]] | ||
[[Category: Mei-p26]] | |||
[[Category: Nhl]] | |||
[[Category: Rna binding protein]] | |||
[[Category: Rna recognition]] | |||
[[Category: Trim-nhl protein]] | |||
Revision as of 10:23, 18 May 2022
Crystal structure of the Mei-P26 NHL domain
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