7ph0: Difference between revisions
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==Structure of light-adapted AsLOV2 Q513L== | ==Structure of light-adapted AsLOV2 Q513L== | ||
<StructureSection load='7ph0' size='340' side='right'caption='[[7ph0]]' scene=''> | <StructureSection load='7ph0' size='340' side='right'caption='[[7ph0]], [[Resolution|resolution]] 0.98Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PH0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PH0 FirstGlance]. <br> | <table><tr><td colspan='2'>[[7ph0]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PH0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PH0 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ph0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ph0 OCA], [https://pdbe.org/7ph0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ph0 RCSB], [https://www.ebi.ac.uk/pdbsum/7ph0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ph0 ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ph0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ph0 OCA], [https://pdbe.org/7ph0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ph0 RCSB], [https://www.ebi.ac.uk/pdbsum/7ph0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ph0 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In nature as in biotechnology, light-oxygen-voltage photoreceptors perceive blue light to elicit spatiotemporally defined cellular responses. Photon absorption drives thioadduct formation between a conserved cysteine and the flavin chromophore. An equally conserved, proximal glutamine processes the resultant flavin protonation into downstream hydrogen-bond rearrangements. Here, we report that this glutamine, long deemed essential, is generally dispensable. In its absence, several light-oxygen-voltage receptors invariably retained productive, if often attenuated, signaling responses. Structures of a light-oxygen-voltage paradigm at around 1 A resolution revealed highly similar light-induced conformational changes, irrespective of whether the glutamine is present. Naturally occurring, glutamine-deficient light-oxygen-voltage receptors likely serve as bona fide photoreceptors, as we showcase for a diguanylate cyclase. We propose that without the glutamine, water molecules transiently approach the chromophore and thus propagate flavin protonation downstream. Signaling without glutamine appears intrinsic to light-oxygen-voltage receptors, which pertains to biotechnological applications and suggests evolutionary descendance from redox-active flavoproteins. | |||
Signal transduction in light-oxygen-voltage receptors lacking the active-site glutamine.,Dietler J, Gelfert R, Kaiser J, Borin V, Renzl C, Pilsl S, Ranzani AT, Garcia de Fuentes A, Gleichmann T, Diensthuber RP, Weyand M, Mayer G, Schapiro I, Moglich A Nat Commun. 2022 May 12;13(1):2618. doi: 10.1038/s41467-022-30252-4. PMID:35552382<ref>PMID:35552382</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7ph0" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Gelfert R]] | [[Category: Gelfert, R]] | ||
[[Category: Moeglich A]] | [[Category: Moeglich, A]] | ||
[[Category: Weyand M]] | [[Category: Weyand, M]] | ||
[[Category: Flavin]] | |||
[[Category: Light-oxygen-voltage]] | |||
[[Category: Optogenetic]] | |||
[[Category: Photoreceptor]] | |||
[[Category: Phototropin]] | |||
[[Category: Signaling protein]] | |||
Revision as of 08:13, 25 May 2022
Structure of light-adapted AsLOV2 Q513L
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