3pge

Structure of sumoylated PCNA

spinqualitylabelsall models 3pge, resolution 2.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3pge is a 2 chain structure with sequence from Atcc 18824 and Baker's yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 3l0x Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [SMT3_YEAST] Not known; suppressor of MIF2 mutations. [PCNA_YEAST] This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involved in DNA repair. Publication Abstract from PubMed Eukaryotic proliferating cell nuclear antigen (PCNA) is a replication accessory protein that functions in DNA replication, repair, and recombination. The various functions of PCNA are regulated by posttranslational modifications including mono-ubiquitylation, which promotes translesion synthesis, and sumoylation, which inhibits recombination. To understand how SUMO modification regulates PCNA, we generated a split SUMO-modified PCNA protein and showed that it supports cell viability and stimulates DNA polymerase delta activity. We then determined its X-ray crystal structure and found that SUMO occupies a position on the back face of the PCNA ring, which is distinct from the position occupied by ubiquitin in the structure of ubiquitin-modified PCNA. We propose that the back of PCNA has evolved to be a site of regulation that can be easily modified without disrupting ongoing reactions on the front of PCNA, such as normal DNA replication. Moreover, these modifications likely allow PCNA to function as a tool belt, whereby proteins can be recruited to the replication machinery via the back of PCNA and be held in reserve until needed. Crystal Structure of SUMO-Modified Proliferating Cell Nuclear Antigen.,Freudenthal BD, Brogie JE, Gakhar L, Kondratick CM, Washington MT J Mol Biol. 2010 Dec 15. PMID:21167178 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Proliferating cell nuclear antigen 3D structures

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