3pic: Difference between revisions

Revision as of 08:40, 25 May 2022

Glucuronoyl Esterase catalytic domain (Cip2_GE) from Hypocrea jecorina

Structural highlights

3pic is a 3 chain structure with sequence from Hypocrea jecorina. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	NAG
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CIP2_HYPJQ] Glucuronoyl esterase which may play a significant role in biomass degradation, as it is considered to disconnect hemicellulose from lignin through the hydrolysis of the ester bond between 4-O-methyl-D-glucuronic acid residues of glucuronoxylans and aromatic alcohols of lignin. Does not hydrolyze substrates of other carbohydrate esterases such as acetylxylan esterase, acetyl esterase and feruloyl esterase.^[1] ^[2]

Publication Abstract from PubMed

The structure of the catalytic domain of glucuronoyl esterase Cip2 from the fungus H. jecorina was determined at a resolution of 1.9 A. This is the first structure of the newly established carbohydrate esterase family 15. The structure has revealed the residues Ser278-His411-Glu301 present in a triad arrangement as the active site. Ser278 is present in the novel consensus sequence GCSRXG reported earlier in the members of CE-15 family. The active site is exposed on the surface of the protein which has implications for the ability of the enzyme to hydrolyze ester bonds of large substrates. Efforts are underway to obtain crystals of Cip2_GE complexed with inhibitor and synthetic substrates. The activity of the glucuronoyl esterase could play a significant role in plant biomass degradation as its expected role is to separate the lignin from hemicelluloses by hydrolysis of the ester bond between 4-O-methyl-D-glucuronic acid moieties of glucuronoxylans and aromatic alcohols of lignin.

Structure of the catalytic domain of glucuronoyl esterase Cip2 from Hypocrea jecorina.,Pokkuluri PR, Duke NE, Wood SJ, Cotta MA, Li XL, Biely P, Schiffer M Proteins. 2011 Aug;79(8):2588-92. doi: 10.1002/prot.23088. Epub 2011 Jun 9. PMID:21661060^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Foreman PK, Brown D, Dankmeyer L, Dean R, Diener S, Dunn-Coleman NS, Goedegebuur F, Houfek TD, England GJ, Kelley AS, Meerman HJ, Mitchell T, Mitchinson C, Olivares HA, Teunissen PJ, Yao J, Ward M. Transcriptional regulation of biomass-degrading enzymes in the filamentous fungus Trichoderma reesei. J Biol Chem. 2003 Aug 22;278(34):31988-97. Epub 2003 Jun 4. PMID:12788920 doi:https://dx.doi.org/10.1074/jbc.M304750200
↑ Li XL, Spanikova S, de Vries RP, Biely P. Identification of genes encoding microbial glucuronoyl esterases. FEBS Lett. 2007 Aug 21;581(21):4029-35. Epub 2007 Jul 26. PMID:17678650 doi:https://dx.doi.org/10.1016/j.febslet.2007.07.041
↑ Pokkuluri PR, Duke NE, Wood SJ, Cotta MA, Li XL, Biely P, Schiffer M. Structure of the catalytic domain of glucuronoyl esterase Cip2 from Hypocrea jecorina. Proteins. 2011 May 16. doi: 10.1002/prot.23088. PMID:21661060 doi:10.1002/prot.23088

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Foreman PK, Brown D, Dankmeyer L, Dean R, Diener S, Dunn-Coleman NS, Goedegebuur F, Houfek TD, England GJ, Kelley AS, Meerman HJ, Mitchell T, Mitchinson C, Olivares HA, Teunissen PJ, Yao J, Ward M. Transcriptional regulation of biomass-degrading enzymes in the filamentous fungus Trichoderma reesei. J Biol Chem. 2003 Aug 22;278(34):31988-97. Epub 2003 Jun 4. PMID:12788920 doi:https://dx.doi.org/10.1074/jbc.M304750200

[2] Li XL, Spanikova S, de Vries RP, Biely P. Identification of genes encoding microbial glucuronoyl esterases. FEBS Lett. 2007 Aug 21;581(21):4029-35. Epub 2007 Jul 26. PMID:17678650 doi:https://dx.doi.org/10.1016/j.febslet.2007.07.041

[3] Pokkuluri PR, Duke NE, Wood SJ, Cotta MA, Li XL, Biely P, Schiffer M. Structure of the catalytic domain of glucuronoyl esterase Cip2 from Hypocrea jecorina. Proteins. 2011 May 16. doi: 10.1002/prot.23088. PMID:21661060 doi:10.1002/prot.23088

[1]

[2]

[3]

@@ Line 1: / Line 1: @@
 ==Glucuronoyl Esterase catalytic domain (Cip2_GE) from Hypocrea jecorina==
-<StructureSection load='3pic' size='340' side='right' caption='[[3pic]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+<StructureSection load='3pic' size='340' side='right'caption='[[3pic]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3pic]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PIC FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3pic]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PIC FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pic OCA], [http://pdbe.org/3pic PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3pic RCSB], [http://www.ebi.ac.uk/pdbsum/3pic PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3pic ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pic OCA], [https://pdbe.org/3pic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pic RCSB], [https://www.ebi.ac.uk/pdbsum/3pic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pic ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CIP2_HYPJQ CIP2_HYPJQ]] Glucuronoyl esterase which may play a significant role in biomass degradation, as it is considered to disconnect hemicellulose from lignin through the hydrolysis of the ester bond between 4-O-methyl-D-glucuronic acid residues of glucuronoxylans and aromatic alcohols of lignin. Does not hydrolyze substrates of other carbohydrate esterases such as acetylxylan esterase, acetyl esterase and feruloyl esterase.<ref>PMID:12788920</ref> <ref>PMID:17678650</ref>
+[[https://www.uniprot.org/uniprot/CIP2_HYPJQ CIP2_HYPJQ]] Glucuronoyl esterase which may play a significant role in biomass degradation, as it is considered to disconnect hemicellulose from lignin through the hydrolysis of the ester bond between 4-O-methyl-D-glucuronic acid residues of glucuronoxylans and aromatic alcohols of lignin. Does not hydrolyze substrates of other carbohydrate esterases such as acetylxylan esterase, acetyl esterase and feruloyl esterase.<ref>PMID:12788920</ref> <ref>PMID:17678650</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 </StructureSection>
 [[Category: Hypocrea jecorina]]
+[[Category: Large Structures]]
 [[Category: Pokkuluri, P R]]
 [[Category: Alpha/beta hydrolase fold]]

3pic: Difference between revisions

Revision as of 08:40, 25 May 2022

Glucuronoyl Esterase catalytic domain (Cip2_GE) from Hypocrea jecorina

Structural highlights

Function

Publication Abstract from PubMed

References

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Proteopedia Page Contributors and Editors (what is this?)

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