3rdy

Crystal Structure of buckwheat trypsin inhibitor rBTI at 1.84 angstrom resolution

spinqualitylabelsall models 3rdy, resolution 1.84Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3rdy is a 1 chain structure with sequence from Common buckwheat. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 3rdz Gene: BWI-1, Edn1 (Common buckwheat) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed BWI-1 (buckwheat trypsin inhibitor), a member of the potato inhibitor I family, suppresses the growth of T-acute lymphoblastic leukemia cells and induces apoptosis in human solid tumor cell lines. Here, we report the crystal structure of rBTI (recombinant buckwheat trypsin inhibitor), a recombinant protein of BWI-1, at 1.84 A resolution and the structure of rBTI in complex with bovine trypsin at 2.26 A resolution. A conformational change of Trp53 at the P(8)' position in rBTI was observed upon its binding to trypsin, which is not seen in other members of the potato inhibitor I family reported previously. The role of the P(8)' residue in the potato inhibitor I family was examined by measuring the association and dissociation rates of four rBTI mutants with different substitutions at the P(2) and P(8)' positions when binding to trypsin. One of the mutants, P44T, was found to be a much stronger inhibitor than wild-type rBTI, with a picomolar (pM) dissociation constant. Our results could provide valuable insights for designing a new rBTI-based antitumor drug in the future. Conformational Changes of rBTI from Buckwheat upon Binding to Trypsin: Implications for the Role of the P(8)' Residue in the Potato Inhibitor I Family.,Wang L, Zhao F, Li M, Zhang H, Gao Y, Cao P, Pan X, Wang Z, Chang W PLoS One. 2011;6(6):e20950. Epub 2011 Jun 15. PMID:21698291 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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