7vx8: Difference between revisions
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==Cryo-EM structure of ATP-bound human very long-chain fatty acid ABC transporter ABCD1== | ==Cryo-EM structure of ATP-bound human very long-chain fatty acid ABC transporter ABCD1== | ||
<StructureSection load='7vx8' size='340' side='right'caption='[[7vx8]]' scene=''> | <StructureSection load='7vx8' size='340' side='right'caption='[[7vx8]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VX8 FirstGlance]. <br> | <table><tr><td colspan='2'>[[7vx8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VX8 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vx8 OCA], [https://pdbe.org/7vx8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vx8 RCSB], [https://www.ebi.ac.uk/pdbsum/7vx8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vx8 ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vx8 OCA], [https://pdbe.org/7vx8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vx8 RCSB], [https://www.ebi.ac.uk/pdbsum/7vx8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vx8 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Human ABC transporter ABCD1 transports very long-chain fatty acids from cytosol to peroxisome for beta-oxidation, dysfunction of which usually causes the X-linked adrenoleukodystrophy (X-ALD). Here, we report three cryogenic electron microscopy structures of ABCD1: the apo-form, substrate- and ATP-bound forms. Distinct from what was seen in the previously reported ABC transporters, the two symmetric molecules of behenoyl coenzyme A (C22:0-CoA) cooperatively bind to the transmembrane domains (TMDs). For each C22:0-CoA, the hydrophilic 3'-phospho-ADP moiety of CoA portion inserts into one TMD, with the succeeding pantothenate and cysteamine moiety crossing the inter-domain cavity, whereas the hydrophobic fatty acyl chain extends to the opposite TMD. Structural analysis combined with biochemical assays illustrates snapshots of ABCD1-mediated substrate transport cycle. It advances our understanding on the selective oxidation of fatty acids and molecular pathology of X-ALD. | |||
Structural basis of substrate recognition and translocation by human very long-chain fatty acid transporter ABCD1.,Chen ZP, Xu D, Wang L, Mao YX, Li Y, Cheng MT, Zhou CZ, Hou WT, Chen Y Nat Commun. 2022 Jun 8;13(1):3299. doi: 10.1038/s41467-022-30974-5. PMID:35676282<ref>PMID:35676282</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7vx8" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Chen | [[Category: Chen, Y X]] | ||
[[Category: Chen | [[Category: Chen, Z P]] | ||
[[Category: Cheng | [[Category: Cheng, M T]] | ||
[[Category: Hou | [[Category: Hou, W T]] | ||
[[Category: Mao | [[Category: Mao, Y X]] | ||
[[Category: Wang L]] | [[Category: Wang, L]] | ||
[[Category: Xu D]] | [[Category: Xu, D]] | ||
[[Category: Yang L]] | [[Category: Yang, L]] | ||
[[Category: Zhou | [[Category: Zhou, C Z]] | ||
[[Category: Abc transporter]] | |||
[[Category: Peroxisome]] | |||
[[Category: Transport protein]] | |||
[[Category: Very long-chain fatty]] | |||
Latest revision as of 09:59, 22 June 2022
Cryo-EM structure of ATP-bound human very long-chain fatty acid ABC transporter ABCD1
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