3rmh

Crystal Structure of yeast telomere protein Cdc13 OB4

spinqualitylabelsall models 3rmh, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3rmh is a 2 chain structure with sequence from Atcc 2001. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: CAGL0G05588g (ATCC 2001) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The budding yeast Cdc13-Stn1-Ten1 complex is crucial for telomere protection and has been proposed to resemble structurally and functionally the RPA complex. The Cdc13 homologues in Candida species are unusually small and lack two conserved domains previously implicated in telomere regulation, thus raising interesting questions concerning the mechanisms and evolution of these proteins. In this report, we show that the unusually small Cdc13 homologue in C. albicans is indeed a regulator of telomere lengths, and that it associates with telomere DNA in vivo. We demonstrated high affinity telomere DNA-binding by C. tropicalis Cdc13 (CtCdc13), and found that dimerization of this protein through its OB4 domain is important for high affinity DNA binding. Interestingly, CtCdc13-DNA complex formation appears to involve primarily recognition of multiple copies of a six-nucleotide element (GGATGT) that is shared by many Candida telomere repeats. We also determined the crystal structure of the OB4 domain of C. glabrata Cdc13, which revealed a novel mechanism of OB fold dimerization. The structure also exhibits marked differences to the C-terminal OB fold of RPA70, thus arguing against a close evolutionary kinship between these two proteins. Our findings provide new insights on the mechanisms and evolution of a critical telomere end binding protein. Analyses of Candida Cdc13 orthologues revealed a novel OB fold dimer arrangement, dimerization-assisted DNA-binding, and substantial structural differences between Cdc13 and RPA70.,Yu EY, Sun J, Lei M, Lue NF Mol Cell Biol. 2011 Oct 24. PMID:22025677 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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