7q8b: Difference between revisions
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==Leishmania major actin filament in ADP-Pi state== | ==Leishmania major actin filament in ADP-Pi state== | ||
<StructureSection load='7q8b' size='340' side='right'caption='[[7q8b]]' scene=''> | <StructureSection load='7q8b' size='340' side='right'caption='[[7q8b]], [[Resolution|resolution]] 3.30Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Q8B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Q8B FirstGlance]. <br> | <table><tr><td colspan='2'>[[7q8b]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Q8B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Q8B FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q8b OCA], [https://pdbe.org/7q8b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q8b RCSB], [https://www.ebi.ac.uk/pdbsum/7q8b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q8b ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q8b OCA], [https://pdbe.org/7q8b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q8b RCSB], [https://www.ebi.ac.uk/pdbsum/7q8b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q8b ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Actin polymerization generates forces for cellular processes throughout the eukaryotic kingdom, but our understanding of the 'ancient' actin turnover machineries is limited. We show that, despite > 1 billion years of evolution, pathogenic Leishmania major parasite and mammalian actins share the same overall fold and co-polymerize with each other. Interestingly, Leishmania harbors a simple actin-regulatory machinery that lacks cofilin 'cofactors', which accelerate filament disassembly in higher eukaryotes. By applying single-filament biochemistry we discovered that, compared to mammalian proteins, Leishmania actin filaments depolymerize more rapidly from both ends, and are severed > 100-fold more efficiently by cofilin. Our high-resolution cryo-EM structures of Leishmania ADP-, ADP-Pi- and cofilin-actin filaments identify specific features at actin subunit interfaces and cofilin-actin interactions that explain the unusually rapid dynamics of parasite actin filaments. Our findings reveal how divergent parasites achieve rapid actin dynamics using a remarkably simple set of actin-binding proteins, and elucidate evolution of the actin cytoskeleton. | |||
Structural basis of rapid actin dynamics in the evolutionarily divergent Leishmania parasite.,Kotila T, Wioland H, Selvaraj M, Kogan K, Antenucci L, Jegou A, Huiskonen JT, Romet-Lemonne G, Lappalainen P Nat Commun. 2022 Jun 15;13(1):3442. doi: 10.1038/s41467-022-31068-y. PMID:35705539<ref>PMID:35705539</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7q8b" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Huiskonen | [[Category: Huiskonen, J T]] | ||
[[Category: Kotila T]] | [[Category: Kotila, T]] | ||
[[Category: Lappalainen P]] | [[Category: Lappalainen, P]] | ||
[[Category: Muniyandi S]] | [[Category: Muniyandi, S]] | ||
[[Category: Actin]] | |||
[[Category: Adp-pi]] | |||
[[Category: Filament]] | |||
[[Category: Parasite]] | |||
[[Category: Structural protein]] | |||
Revision as of 07:33, 29 June 2022
Leishmania major actin filament in ADP-Pi state
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