7shg: Difference between revisions
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==Polysaccharide ribofuranosyl transferase from Thermobacillus composti== | ==Polysaccharide ribofuranosyl transferase from Thermobacillus composti== | ||
<StructureSection load='7shg' size='340' side='right'caption='[[7shg]]' scene=''> | <StructureSection load='7shg' size='340' side='right'caption='[[7shg]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SHG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SHG FirstGlance]. <br> | <table><tr><td colspan='2'>[[7shg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SHG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SHG FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7shg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7shg OCA], [https://pdbe.org/7shg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7shg RCSB], [https://www.ebi.ac.uk/pdbsum/7shg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7shg ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7shg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7shg OCA], [https://pdbe.org/7shg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7shg RCSB], [https://www.ebi.ac.uk/pdbsum/7shg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7shg ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Bacterial surface polysaccharides are assembled by glycosyltransferase enzymes that typically use sugar nucleotide or polyprenyl-monophosphosugar activated donors. Characterized representatives exist for many monosaccharides but neither the donor nor the corresponding glycosyltransferases have been definitively identified for ribofuranose residues found in some polysaccharides. Klebsiella pneumoniae O-antigen polysaccharides provided prototypes to identify dual-domain ribofuranosyltransferase proteins catalyzing a two-step reaction sequence. Phosphoribosyl-5-phospho-D-ribosyl-alpha-1-diphosphate serves as the donor for a glycan acceptor-specific phosphoribosyl transferase (gPRT), and a more promiscuous phosphoribosyl-phosphatase (PRP) then removes the residual 5'-phosphate. The 2.5-A resolution crystal structure of a dual-domain ribofuranosyltransferase ortholog from Thermobacillus composti revealed a PRP domain that conserves many features of the phosphatase members of the haloacid dehalogenase family, and a gPRT domain that diverges substantially from all previously characterized phosphoribosyl transferases. The gPRT represents a new glycosyltransferase fold conserved in the most abundant ribofuranosyltransferase family. | |||
The biosynthetic origin of ribofuranose in bacterial polysaccharides.,Kelly SD, Williams DM, Nothof JT, Kim T, Lowary TL, Kimber MS, Whitfield C Nat Chem Biol. 2022 May;18(5):530-537. doi: 10.1038/s41589-022-01006-6. Epub 2022, Apr 7. PMID:35393575<ref>PMID:35393575</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7shg" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Kelly | [[Category: Kelly, S D]] | ||
[[Category: Kimber | [[Category: Kimber, M S]] | ||
[[Category: Glycosyltransferase]] | |||
[[Category: Phosphatase]] | |||
[[Category: Ribofuranose]] | |||
[[Category: Transferase]] | |||
Revision as of 07:34, 29 June 2022
Polysaccharide ribofuranosyl transferase from Thermobacillus composti
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