3j7t

Calcium atpase structure with two bound calcium ions determined by electron crystallography of thin 3D crystals

spinqualitylabelsall models 3j7t, resolution 3.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3j7t is a 1 chain structure with sequence from Oryctolagus cuniculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CA, NA Related: 1su4, 3j7u Activity: Calcium-transporting ATPase, with EC number 3.6.3.8 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [AT2A1_RABIT] This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction (By similarity). Publication Abstract from PubMed Membrane proteins and macromolecular complexes often yield crystals too small or too thin for even the modern synchrotron X-ray beam. Electron crystallography could provide a powerful means for structure determination with such undersized crystals, as protein atoms diffract electrons four to five orders of magnitude more strongly than they do X-rays. Furthermore, as electron crystallography yields Coulomb potential maps rather than electron density maps, it could provide a unique method to visualize the charged states of amino acid residues and metals. Here we describe an attempt to develop a methodology for electron crystallography of ultrathin (only a few layers thick) 3D protein crystals and present the Coulomb potential maps at 3.4-A and 3.2-A resolution, respectively, obtained from Ca(2+)-ATPase and catalase crystals. These maps demonstrate that it is indeed possible to build atomic models from such crystals and even to determine the charged states of amino acid residues in the Ca(2+)-binding sites of Ca(2+)-ATPase and that of the iron atom in the heme in catalase. Electron crystallography of ultrathin 3D protein crystals: atomic model with charges.,Yonekura K, Kato K, Ogasawara M, Tomita M, Toyoshima C Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3368-73. doi:, 10.1073/pnas.1500724112. Epub 2015 Feb 17. PMID:25730881 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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