3sji

crystal structure of CVA16 3C in complex with Rupintrivir (AG7088)

spinqualitylabelsall models 3sji, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3sji is a 1 chain structure with sequence from Cv-a16. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: AG7, NA Related: 1cqq, 2zty, 1l1n, 3sj8, 3sj9, 3sjk, 3sjo Gene: 3C (CV-A16) Activity: Picornain 3C, with EC number 3.4.22.28 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) are the major causative agents of hand foot and mouth disease (HFMD) which is prevalent in Asia. Thus far there are no prophylactic or therapeutic measures against HFMD. The 3C proteases from EV71 and CVA16 play important roles in viral replication and are therefore ideal drug targets. By using biochemical, mutational and structural approaches, we broadly characterized both proteases. A series of high-resolution structures of the free or substrate-bound enzymes were solved. These structures, together with our cleavage specificity assay, well explain the marked substrate preferences of both proteases for particular P4, P1 and P1' residue types, as well as their relative malleability for P2 amino acid. More importantly, the complex structures of EV71 and CVA16 3Cs with Rupintrivir, a specific human rhinovirus (HRV) 3C protease inhibitor, were solved. These structures reveal a half-closed S2 subsite and a size-reduced S1' subsite that limit the access of the P1' group of Rupitrivir to both enzymes, explaining the reported low inhibition activity of the compound toward EV71 and CVA16. In conclusion, the detailed characterization of both proteases in this study could direct us for a proposal of rational design of EV71/CVA16 3C inhibitors. Enterovirus 71 and Coxsackievirus A16 3C proteases: binding to Rupintrivir and their substrate, and anti-HFMD drug design.,Lu G, Qi J, Chen Z, Xu X, Gao F, Lin D, Qian W, Liu H, Jiang H, Yan J, Gao GF J Virol. 2011 Jul 27. PMID:21795339 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Virus protease 3D structures

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