7o42: Difference between revisions
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==TrwK/VirB4unbound trimer of dimers complex (with Hcp1) from the R388 type IV secretion system determined by cryo-EM.== | ==TrwK/VirB4unbound trimer of dimers complex (with Hcp1) from the R388 type IV secretion system determined by cryo-EM.== | ||
<StructureSection load='7o42' size='340' side='right'caption='[[7o42]]' scene=''> | <StructureSection load='7o42' size='340' side='right'caption='[[7o42]], [[Resolution|resolution]] 4.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O42 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O42 FirstGlance]. <br> | <table><tr><td colspan='2'>[[7o42]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O42 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O42 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o42 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o42 OCA], [https://pdbe.org/7o42 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o42 RCSB], [https://www.ebi.ac.uk/pdbsum/7o42 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o42 ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o42 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o42 OCA], [https://pdbe.org/7o42 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o42 RCSB], [https://www.ebi.ac.uk/pdbsum/7o42 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o42 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Bacterial conjugation is the fundamental process of unidirectional transfer of DNAs, often plasmid DNAs, from a donor cell to a recipient cell(1). It is the primary means by which antibiotic resistance genes spread among bacterial populations(2,3). In Gram-negative bacteria, conjugation is mediated by a large transport apparatus-the conjugative type IV secretion system (T4SS)-produced by the donor cell and embedded in both its outer and inner membranes. The T4SS also elaborates a long extracellular filament-the conjugative pilus-that is essential for DNA transfer(4,5). Here we present a high-resolution cryo-electron microscopy (cryo-EM) structure of a 2.8 megadalton T4SS complex composed of 92 polypeptides representing 8 of the 10 essential T4SS components involved in pilus biogenesis. We added the two remaining components to the structural model using co-evolution analysis of protein interfaces, to enable the reconstitution of the entire system including the pilus. This structure describes the exceptionally large protein-protein interaction network required to assemble the many components that constitute a T4SS and provides insights on the unique mechanism by which they elaborate pili. | |||
Cryo-EM structure of a type IV secretion system.,Mace K, Vadakkepat AK, Redzej A, Lukoyanova N, Oomen C, Braun N, Ukleja M, Lu F, Costa TRD, Orlova EV, Baker D, Cong Q, Waksman G Nature. 2022 Jun 22. pii: 10.1038/s41586-022-04859-y. doi:, 10.1038/s41586-022-04859-y. PMID:35732732<ref>PMID:35732732</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7o42" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Lukoyanova N]] | [[Category: Lukoyanova, N]] | ||
[[Category: Mace K]] | [[Category: Mace, K]] | ||
[[Category: Vadakkepat | [[Category: Vadakkepat, A K]] | ||
[[Category: Waksman G]] | [[Category: Waksman, G]] | ||
[[Category: Atpase]] | |||
[[Category: Bacterial secretion]] | |||
[[Category: Conjugation]] | |||
[[Category: Hcp]] | |||
[[Category: Inner membrane]] | |||
[[Category: Inner membrane complex]] | |||
[[Category: Membrane protein]] | |||
[[Category: Protein complex]] | |||
[[Category: R388 plasmid]] | |||
[[Category: Secretion]] | |||
[[Category: Secretion system]] | |||
[[Category: T4ss]] | |||
[[Category: Trwk]] | |||
[[Category: Type 4 secretion system]] | |||
[[Category: Type iv secretion system]] | |||
[[Category: Virb4]] | |||
Revision as of 16:22, 6 July 2022
TrwK/VirB4unbound trimer of dimers complex (with Hcp1) from the R388 type IV secretion system determined by cryo-EM.
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