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==Crystal structure of human bleomycin hydrolase C73S mutant==
==Crystal structure of human bleomycin hydrolase C73S mutant==
<StructureSection load='7v5t' size='340' side='right'caption='[[7v5t]]' scene=''>
<StructureSection load='7v5t' size='340' side='right'caption='[[7v5t]], [[Resolution|resolution]] 3.25&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7V5T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7V5T FirstGlance]. <br>
<table><tr><td colspan='2'>[[7v5t]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7V5T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7V5T FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v5t OCA], [https://pdbe.org/7v5t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v5t RCSB], [https://www.ebi.ac.uk/pdbsum/7v5t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v5t ProSAT]</span></td></tr>
</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Bleomycin_hydrolase Bleomycin hydrolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.40 3.4.22.40] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v5t OCA], [https://pdbe.org/7v5t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v5t RCSB], [https://www.ebi.ac.uk/pdbsum/7v5t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v5t ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[[https://www.uniprot.org/uniprot/BLMH_HUMAN BLMH_HUMAN]] The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Human bleomycin hydrolase (hBH) catalyzes deamidation of the anticancer drug bleomycins (BLM). This enzyme is involved in BLM detoxification and drug resistance. Herein, we report the putative BLM-binding site and catalytic mechanism of hBH. The crystal structures and biochemical studies suggest that hBH cleaves its C-terminal residue without significant preference for the type of amino acid, and therefore can accordingly accommodate the beta-aminoalanine amide moiety of BLM for deamidation. Interestingly, hBH is capable of switching from a cysteine protease to a serine protease that is unable to cleave the secondary amide of hBH C-terminus but reacts with the primary amide of BLMs.
The Structure-Function Relationship of Human Bleomycin Hydrolase: Mutation of a Cysteine Protease into a Serine Protease.,Zheng YZ, Cui J, Wang YL, Huang SJ, Lin EC, Huang SC, Rudolf JD, Yan X, Chang CY Chembiochem. 2022 Jun 20;23(12):e202200186. doi: 10.1002/cbic.202200186. Epub, 2022 May 5. PMID:35467071<ref>PMID:35467071</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7v5t" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bleomycin hydrolase]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Chang, CY, Zheng, YZ, Huang, SJ, Wang, YL, Toh, SI, Lin, EC]]
[[Category: Chang, C Y]]
[[Category: Huang, S J]]
[[Category: Lin, E C]]
[[Category: Toh, S I]]
[[Category: Wang, Y L]]
[[Category: Zheng, Y Z]]
[[Category: Cysteine protease]]
[[Category: Hydrolase]]
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