7dtr: Difference between revisions
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==Structure of an AcrIF protein== | ==Structure of an AcrIF protein== | ||
<StructureSection load='7dtr' size='340' side='right'caption='[[7dtr]]' scene=''> | <StructureSection load='7dtr' size='340' side='right'caption='[[7dtr]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DTR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DTR FirstGlance]. <br> | <table><tr><td colspan='2'>[[7dtr]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DTR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DTR FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dtr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dtr OCA], [https://pdbe.org/7dtr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dtr RCSB], [https://www.ebi.ac.uk/pdbsum/7dtr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dtr ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dtr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dtr OCA], [https://pdbe.org/7dtr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dtr RCSB], [https://www.ebi.ac.uk/pdbsum/7dtr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dtr ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
CRISPR-Cas systems are prokaryotic adaptive immune systems and phages use anti-CRISPR proteins (Acrs) to counteract these systems. Here, we report the structures of AcrIF24 and its complex with the crRNA-guided surveillance (Csy) complex. The HTH motif of AcrIF24 can bind the Acr promoter region and repress its transcription, suggesting its role as an Aca gene in self-regulation. AcrIF24 forms a homodimer and further induces dimerization of the Csy complex. Apart from blocking the hybridization of target DNA to the crRNA, AcrIF24 also induces the binding of non-sequence-specific dsDNA to the Csy complex, similar to AcrIF9, although this binding seems to play a minor role in AcrIF24 inhibitory capacity. Further structural and biochemical studies of the Csy-AcrIF24-dsDNA complexes and of AcrIF24 mutants reveal that the HTH motif of AcrIF24 and the PAM recognition loop of the Csy complex are structural elements essential for this non-specific dsDNA binding. Moreover, AcrIF24 and AcrIF9 display distinct characteristics in inducing non-specific DNA binding. Together, our findings highlight a multifunctional Acr and suggest potential wide distribution of Acr-induced non-specific DNA binding. | |||
Insights into the inhibition of type I-F CRISPR-Cas system by a multifunctional anti-CRISPR protein AcrIF24.,Yang L, Zhang L, Yin P, Ding H, Xiao Y, Zeng J, Wang W, Zhou H, Wang Q, Zhang Y, Chen Z, Yang M, Feng Y Nat Commun. 2022 Apr 11;13(1):1931. doi: 10.1038/s41467-022-29581-1. PMID:35411005<ref>PMID:35411005</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7dtr" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Peipei Y]] | [[Category: Peipei, Y]] | ||
[[Category: Yue F]] | [[Category: Yue, F]] | ||
[[Category: Hth motif]] | |||
[[Category: Protein interaction]] | |||
[[Category: Viral protein]] | |||
