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==Structure of the Listeria monocytogenes GlnR-DNA complex to 3.45 Angstrom==
==Structure of the Listeria monocytogenes GlnR-DNA complex to 3.45 Angstrom==
<StructureSection load='7tec' size='340' side='right'caption='[[7tec]]' scene=''>
<StructureSection load='7tec' size='340' side='right'caption='[[7tec]], [[Resolution|resolution]] 3.45&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TEC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TEC FirstGlance]. <br>
<table><tr><td colspan='2'>[[7tec]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TEC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TEC FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tec OCA], [https://pdbe.org/7tec PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tec RCSB], [https://www.ebi.ac.uk/pdbsum/7tec PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tec ProSAT]</span></td></tr>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tec OCA], [https://pdbe.org/7tec PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tec RCSB], [https://www.ebi.ac.uk/pdbsum/7tec PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tec ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
How bacteria sense and respond to nitrogen levels are central questions in microbial physiology. In Gram-positive bacteria, nitrogen homeostasis is controlled by an operon encoding glutamine synthetase (GS), a dodecameric machine that assimilates ammonium into glutamine, and the GlnR repressor. GlnR detects nitrogen excess indirectly by binding glutamine-feedback-inhibited-GS (FBI-GS), which activates its transcription-repression function. The molecular mechanisms behind this regulatory circuitry, however, are unknown. Here we describe biochemical and structural analyses of GS and FBI-GS-GlnR complexes from pathogenic and non-pathogenic Gram-positive bacteria. The structures show FBI-GS binds the GlnR C-terminal domain within its active-site cavity, juxtaposing two GlnR monomers to form a DNA-binding-competent GlnR dimer. The FBI-GS-GlnR interaction stabilizes the inactive GS conformation. Strikingly, this interaction also favors a remarkable dodecamer to tetradecamer transition in some GS, breaking the paradigm that all bacterial GS are dodecamers. These data thus unveil unique structural mechanisms of transcription and enzymatic regulation.
Molecular dissection of the glutamine synthetase-GlnR nitrogen regulatory circuitry in Gram-positive bacteria.,Travis BA, Peck JV, Salinas R, Dopkins B, Lent N, Nguyen VD, Borgnia MJ, Brennan RG, Schumacher MA Nat Commun. 2022 Jul 1;13(1):3793. doi: 10.1038/s41467-022-31573-0. PMID:35778410<ref>PMID:35778410</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7tec" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Schumacher, MA, Brennan, RG]]
[[Category: Brennan, R G]]
[[Category: Schumacher, M A]]
[[Category: Dna binding protein]]
[[Category: Dna binding protein-dna complex]]
[[Category: Glnr]]
[[Category: Glnra]]
[[Category: Glutamine synthetase]]
[[Category: Listeria]]
[[Category: Repressor]]
[[Category: Transcription]]
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