7aov: Difference between revisions
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==Crystal Structure of a TRPM2 Domain== | ==Crystal Structure of a TRPM2 Domain== | ||
<StructureSection load='7aov' size='340' side='right'caption='[[7aov]]' scene=''> | <StructureSection load='7aov' size='340' side='right'caption='[[7aov]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AOV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AOV FirstGlance]. <br> | <table><tr><td colspan='2'>[[7aov]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AOV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AOV FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aov OCA], [https://pdbe.org/7aov PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aov RCSB], [https://www.ebi.ac.uk/pdbsum/7aov PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aov ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aov OCA], [https://pdbe.org/7aov PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aov RCSB], [https://www.ebi.ac.uk/pdbsum/7aov PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aov ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[[https://www.uniprot.org/uniprot/TRPM2_DANRE TRPM2_DANRE]] Nonselective, voltage-independent cation channel that mediates Ca(2+) influx, leading to increased cytoplasmic Ca(2+) levels. Functions as ligand-gated ion channel. Binding of ADP-ribose to the cytoplasmic N-terminal region causes a conformation change; the channel is primed but still requires Ca(2+) binding to trigger channel opening.<ref>PMID:30250252</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Transient receptor potential melastatin 2 (TRPM2) is a Ca(2+) -permeable, nonselective cation channel involved in diverse physiological processes such as immune response, apoptosis, and body temperature sensing. TRPM2 is activated by ADP-ribose (ADPR) and 2'-deoxy-ADPR in a Ca(2+) -dependent manner. While two distinct binding sites exist for ADPR that exert different functions dependent on the species, the involvement of either binding site regarding the superagonistic effect of 2'-deoxy-ADPR is not clear yet. Here, we report the crystal structure of the MHR1/2 domain of TRPM2 from zebrafish (Danio rerio), and show that both ligands bind to this domain and activate the channel. We identified a so far unrecognized Zn(2+) -binding domain that was not resolved in previous cryo-EM structures and that is conserved in most TRPM channels. In combination with patch clamp experiments we comprehensively characterize the effect of the Zn(2+) -binding domain on TRPM2 activation. Our results provide insight into a conserved motif essential for structural integrity and channel activity. | |||
The crystal structure of TRPM2 MHR1/2 domain reveals a conserved Zn(2+) -binding domain essential for structural integrity and channel activity.,Sander S, Pick J, Gattkowski E, Fliegert R, Tidow H Protein Sci. 2022 Jun;31(6):e4320. doi: 10.1002/pro.4320. PMID:35634784<ref>PMID:35634784</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7aov" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Sander S]] | [[Category: Sander, S]] | ||
[[Category: Tidow H]] | [[Category: Tidow, H]] | ||
[[Category: Signaling protein]] | |||
[[Category: Trpm2]] | |||
Revision as of 06:29, 18 August 2022
Crystal Structure of a TRPM2 Domain
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